追觅 - 2026-01-17

生成时间: 2026−01-17 22:37

分析文章数量: 134

1 Single-Cell Profiling of ANKRD26 Thrombocytopenia Reveals Progenitor Expansion and Polyploid Apoptosis via JUNB-p21

推荐理由: 该研究通过直接分析患者骨髓样本，克服了罕见病研究的核心难点，为ANKRD26相关血小板减少症的发病机制提供了关键的直接证据，对理解巨核细胞发育及该病的临床诊疗具有重要参考价值。

摘要: ANKRD26-related thrombocytopenia (THC2) is a rare inherited platelet disorder caused by germline variants in the 5′ untranslated region (UTR) of ANKRD26. While prior studies using in vitro models or isolated case reports have suggested impaired megakaryopoiesis as a central mechanism, detailed insights have remained elusive-primarily due to the rarity, fragility, and heterogeneity of megakaryocytes. Here, we present a comprehensive, cross-validated analysis of bone marrow samples from four…

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2 CBX3 confers ferroptosis resistance during blood-borne metastasis

推荐理由: 该研究利用单细胞测序与微流控技术

摘要: The survival mechanisms of circulating tumor cells (CTCs) remain poorly understood while these rare cell populations transit through the blood stream and colonize distant organs including the brain. Using single-cell RNA-seq of microfluidically-enriched CTCs and patient-matched brain metastatic tumor cells of lung adenocarcinoma (LUAD), we revealed CTC-selective upregulation of core gene signatures associated with ferroptosis. The malignancy of CTCs was confirmed through comparative genomic…

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3 Inferring chromatin architecture at a single locus through probabilistic in situ DNA localization

推荐理由: 该研究提出的PLOTTED技术为染色质空间构象研究提供了创新的高分辨率方法，对解析血液疾病中增强子-启动子互作异常具有重要工具价值。

摘要: Chromatin conformation is thought to be critical for enhancer function, but its dynamic, nanoscale organization is difficult to measure directly. Here we introduce PLOTTED (Probabilistic Localization of Oligopaint Tagged Target Element Distances), an integrated imaging and computational framework that infers chromatin architecture from targeted high-resolution imaging of cis-regulatory modules (CRMs). PLOTTED generates spatial distance distributions between DNA loci, enabling quantitative…

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4 Axes of biological variation in diffuse large B cell lymphoma

推荐理由: 该研究首次在单细胞水平系统解析DLBCL的遗传亚型特征，其构建的分子标签为淋巴瘤精准分型与靶向治疗提供了重要参考依据。

摘要: Genetic and gene expression subtypes of diffuse large B cell lymphoma (DLBCL) have been defined using bulk tumor analysis. To explore their biology, we derived single-cell RNA and ATAC sequencing data from 103 DLBCL biopsies and identified malignant B cells by their non-diploid DNA copy number profiles. Using malignant B cell gene expression, we developed and validated signatures of each DLBCL genetic subtype, revealing their distinctive characters. Most biopsies had genetic subclones, defined…

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5 Axes of biological variation in diffuse large B cell lymphoma

推荐理由: 该研究采用前沿的单细胞多组学技术对弥漫大B细胞淋巴瘤进行分子分型探索，为血液肿瘤的精准诊断和靶向治疗提供了重要的生物学依据，对血液科临床与科研具有较高参考价值。

摘要: Genetic and gene expression subtypes of diffuse large B cell lymphoma (DLBCL) have been defined using bulk tumor analysis. To explore their biology, we derived single-cell RNA and ATAC sequencing data from 103 DLBCL biopsies and identified malignant B cells by their non-diploid DNA copy number profiles. Using malignant B cell gene expression, we developed and validated signatures of each DLBCL genetic subtype, revealing their distinctive characters. Most biopsies had genetic subclones, defined…

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6 CAR T cells: the missing piece needed to improve outcomes for children with cancer?

推荐理由: 该文章聚焦于CAR-T细胞疗法在儿童B-ALL治疗中的突破性进展，并探讨其向其他儿科癌症拓展的前景，对血液肿瘤研究和临床治疗具有重要的前沿参考价值。

摘要: B cell acute lymphoblastic leukemia (B-ALL) is the most common cancer of childhood. Outcomes for B-ALL have steadily improved over the last five decades, most recently due to impressive activity of chimeric antigen receptor modified T (CAR-T) cells and bispecific antibodies targeting CD19. In contrast, progress against other pediatric cancers has largely stalled. Significant academic effort is underway to expand the reach of CAR T cell therapy in pediatric cancer beyond B-ALL to other…

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7 Global access to commercial CAR T cells: A cross-sectional study of health technology assessment in the G20

推荐理由: 该文章聚焦CAR-T细胞疗法在血液恶性肿瘤中的临床应用与可及性挑战，既体现了前沿治疗价值，又深入探讨了产业化与卫生技术评估对患者获益的实际影响，对血液科临床与政策研究均有重要参考意义。

摘要: Chimeric antigen receptor T-cells (CAR T) are a major treatment advance for many patients with haematological malignancies, especially those with disease that has relapsed or is refractory to chemotherapy. However, currently approved commercial CAR T-cells require highly specialised manufacturing processes that contribute to high costs and limit their widespread use. In many countries, positive reimbursement recommendations by health technology assessment (HTA) bodies enable patient access to…

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8 Targeting Lactate Metabolism in Cancer Therapy: The Mechanism of Lactylation Modification and Prospects of Therapeutic Strategies

推荐理由: 该文章深入探讨了乳酸在肿瘤代谢重编程和表观遗传调控中的作用，为血液系统恶性肿瘤（如白血病）的耐药机制研究提供了新的代谢视角和潜在治疗靶点，具有较高的借鉴价值。

摘要: Tumor cells preferentially engage in aerobic glycolysis (the Warburg effect) despite normoxic conditions, manifesting in pathological glucose avidity and excessive lactate accu-mulation relative to adjacent normal tissues. Beyond serving as a glycolytic endpoint, lactate acts as a versatile oncometabolite. It epigenetically reinforces multidrug resistance by remod-eling the extracellular microenvironment and driving transcriptional reprogramming via intra-cellular lactylation. Mechanistic…

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9 Targeting Lactate Metabolism in Cancer Therapy: The Mechanism of Lactylation Modification and Prospects of Therapeutic Strategies

推荐理由: 该文章深入探讨了乳酸在肿瘤代谢重编程及多药耐药中的关键作用，其揭示的乳酸化修饰机制为血液系统恶性肿瘤（如淋巴瘤、白血病）的靶向治疗和耐药逆转提供了新的理论依据和潜在干预策略。

摘要: Tumor cells preferentially engage in aerobic glycolysis (the Warburg effect) despite normoxic conditions, manifesting in pathological glucose avidity and excessive lactate accu-mulation relative to adjacent normal tissues. Beyond serving as a glycolytic endpoint, lactate acts as a versatile oncometabolite. It epigenetically reinforces multidrug resistance by remod-eling the extracellular microenvironment and driving transcriptional reprogramming via intra-cellular lactylation. Mechanistic…

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10 Tmem110 regulates the conformation of TRPML1 to maintain endolysosomal homeostasis and prevent mitochondrial DNA leakage and pathological self-DNA processing

推荐理由: 该研究揭示了TMEM110调控循环中线粒体清除的新机制，为理解创伤后全身性炎症及自身免疫反应提供了关键视角，对血液疾病中细胞碎片清除异常及相关免疫病理具有重要借鉴意义。

摘要: The mechanisms by which phagocytes handle large quantities of internalized organelles, such as mitochondria released during tissue injury, remain unclear. Here we show that the endoplasmic reticulum transmembrane regulator TMEM110 is a key determinant of disease severity in traumatic brain injury-associated multiple organ dysfunction. Loss of TMEM110 impairs the clearance of mitochondria aberrantly released into the circulation, leading to heightened autoimmune-mediated tissue injury and…

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11 Structural mechanisms for inhibition and activation of human small-conductance Ca<sup>2+</sup>-activated potassium channel SK2

推荐理由: 该研究通过高分辨率冷冻电镜技术揭示了SK2通道与多种调节剂的结合机制，为针对血液疾病（如镰状细胞贫血）相关的离子通道药物设计提供了关键结构基础。

摘要: The small-conductance calcium-activated potassium (SK1-3 or K(Ca)2) channels regulate the intrinsic excitability and firing frequency of excitable cells. SK channels are modulated by a variety of distinct modulators; however, the underlying mechanisms remain elusive. Here, we present four cryoelectron microscopy structures of the human SK2-calmodulin complex bound with apamin, UCL1684, AP30663, and CAD-1883, elucidating their distinct binding sites and regulatory mechanisms. Apamin and UCL1684…

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12 Strong constitutive NF-κB signaling in B cells drives SLL/CLL-like lymphomagenesis and overcomes microenvironmental dependencies

推荐理由: 该研究通过精确的基因工程小鼠模型，直接揭示了IKK2激酶活性水平与B细胞淋巴瘤发生的剂量依赖性关系，为靶向NF-κB通路的淋巴瘤治疗策略提供了关键的机制依据，对血液肿瘤研究者具有高度参考价值。

摘要: Aberrant activation of NF-κB transcription factors is a hallmark of human lymphomas. Most lymphoma-intrinsic as well as microenvironment-induced NF-κB activation occurs upstream of the key kinase IKK2, therefore affecting additional pathways. Here, we show that canonical NF-κB signaling in mouse B cells, induced through the expression of one or two copies of a constitutively active IKK2 variant, dose-dependently drives lymphomagenesis. The observed phenotype and stereotypic B cell receptor…

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13 BACH2 regulates T cell lineage state to enhance CAR T cell function

推荐理由: 该研究揭示了CAR-T细胞中41BB结构域通过诱导BACH2转录因子调控T细胞干性与耗竭的关键机制，为优化CAR-T疗法、提升血液肿瘤治疗效果提供了重要的理论依据。

摘要: Nearly all chimeric antigen receptors (CARs) signal in the absence of antigen, referred to as ‘tonic signaling’. Tonic signaling of CARs containing 41BB domains enhances T cell fitness and function, in contrast to the exhaustion driven by CD28-containing CARs. Here we show that 41BB induces BACH2, a transcriptional regulator that directs stem and memory programs. Overexpression of BACH2 successfully prevented exhaustion but locked CAR T cells in a quiescent state. We linked BACH2 to a…

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14 Strong constitutive NF-κB signaling in B cells drives SLL/CLL-like lymphomagenesis and overcomes microenvironmental dependencies

推荐理由: 该研究通过精确调控IKK2活性，直接揭示了NF-κB信号在B细胞淋巴瘤发生中的剂量依赖性作用，为淋巴瘤的靶向治疗提供了关键机制依据。

摘要: Aberrant activation of NF-κB transcription factors is a hallmark of human lymphomas. Most lymphoma-intrinsic as well as microenvironment-induced NF-κB activation occurs upstream of the key kinase IKK2, therefore affecting additional pathways. Here, we show that canonical NF-κB signaling in mouse B cells, induced through the expression of one or two copies of a constitutively active IKK2 variant, dose-dependently drives lymphomagenesis. The observed phenotype and stereotypic B cell receptor…

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15 Robust and interpretable prediction of gene markers and cell types from spatial transcriptomics data

推荐理由: 该研究提出的STimage工具将常规H&E图像与空间转录组学结合，创新性强，对于血液肿瘤微环境等研究具有很高的可借鉴价值，能显著提升病理分析的效率和深度。

摘要: Spatial transcriptomics (ST) links tissue morphology with gene expression values, opening new avenues for digital pathology. Deep learning models are used to predict gene expression or classify cell types directly from images, offering significant clinical potential but still requiring improvements in interpretability and robustness. We present STimage as a comprehensive suite of models to predict spatial gene expression and classify cell types directly from standard H&amp;E images. STimage enhances…

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16 Endothelial FUNDC1 regulates metabolic reprogramming and the obesity-diabetes transition through the SIRT3/GATA2/endothelin-1 axis

推荐理由: 该研究创新性地揭示了内皮细胞线粒体蛋白FUNDC1在代谢应激下的调控机制，为理解肥胖/糖尿病相关血管病变及血液疾病（如血栓形成、血管炎症）提供了新的分子靶点和实验依据，具有显著的跨疾病参考价值。

摘要: Endothelial cell (EC) dysfunction is a hallmark of obesity and Type 2 diabetes mellitus (T2DM), yet the mechanisms linking vascular stress to systemic metabolic diseases remain unclear. Here, we investigated the role of the mitochondrial protein FUN14 domain-containing 1 (FUNDC1) in EC under nutritional overload. Using high-fat diet (HFD)-fed EC-specific Fundc1 knockout mice, human umbilical vein ECs, primary ECs, and vascular tissues from patients with obese/T2DM, we find that endothelial…

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17 Identification of thermotolerant non-canonical PAMs for robust one-pot CRISPR-Cas12a detection

推荐理由: 该研究通过温度优化显著扩展了CRISPR-Cas12a系统的PAM识别范围，为血液病基因编辑治疗和分子诊断提供了更灵活的技术方案，对血红蛋白病等遗传性血液疾病的研究具有直接借鉴价值。

摘要: The canonical PAM site TTTV (where V = A, G, or C) is widely used in the design of CRISPR-Cas12a systems for both genome editing and diagnostic applications. Although several non-canonical protospacer-adjacent motifs (PAM) have been identified, they generally exhibit weak Cas12a cleavage activity. In this study, we find that increasing the reaction temperature to 45 °C or higher allows the identification of numerous non-canonical PAMs with trans-cleavage activity comparable to that of canonical…

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18 Epoxy-oxylipins direct monocyte fate in inflammatory resolution in humans

推荐理由: 该研究首次在人体急性炎症模型中系统揭示了环氧氧化脂及其代谢物的动态变化，为血液炎症相关疾病（如脓毒症、自身免疫性血液病）的机制研究与生物标志物开发提供了新的思路和实验依据。

摘要: The role of cytochrome P450-derived epoxy-oxylipins and their metabolites in human inflammation and resolution is unknown. We report that epoxy-oxylipins are present in blood of healthy, male volunteers at baseline and following intradermal injection of UV-killed Escherichia coli, an experimental model of acute resolving inflammation. At the site of inflammation, cytochrome P450s and epoxide hydrolase (EH) isoforms, which catabolise oxylipins to corresponding diols, are differentially…

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19 Hexokinase detachment from mitochondria drives the Warburg effect to support compartmentalized ATP production

推荐理由: 该研究通过CRISPR筛选揭示了HK2在血浆样培养基中对癌细胞生长的重要性，为血液肿瘤（如白血病）的代谢靶向治疗提供了新的潜在方向，对血液病研究具有较高的借鉴价值。

摘要: Hexokinase (HK) catalyses the phosphorylation of glucose to glucose 6-phosphate, marking the first step of glucose metabolism. Most cancer cells co-express two homologous HK isoforms, HK1 and HK2, which can each bind the outer mitochondrial membrane (OMM). CRISPR screens performed across hundreds of cancer cell lines indicate that both isoforms are dispensable for growth in conventional culture media. By contrast, HK2 deletion impaired cell growth in human plasma-like medium. Here we show that…

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20 PU.1-Activated Genomic Regions Define Low-risk MDS Subsets Characterized by Immune Dysregulation and Disease Progression

推荐理由: 该研究通过多组学方法前瞻性分析MDS进展的基因组调控机制，样本量大、技术前沿，对理解MDS向白血病转化及临床风险分层具有重要参考价值。

摘要: Myelodysplastic syndromes (MDS) are heterogeneous myeloid neoplasms with an increased risk of progression to secondary acute myeloid leukemia (sAML). This study investigates the genomic correlates of disease progression in MDS by profiling active genomic regulatory regions and their transcriptional impact through H3K27ac ChIP-seq and RNA-seq analysis on CD34+ bone marrow progenitors cells isolated from a prospective cohort of 86 and 357 patients, respectively. Our analysis revealed distinct…

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21 MicroRNAs in oncology: a translational perspective in the era of AI

推荐理由: 该文章系统梳理了microRNA从基础生物学发现到白血病等血液肿瘤临床研究的演进历程，对理解血液疾病发病机制和开发新型诊疗策略具有重要参考价值。

摘要: Over the past three decades, knowledge of microRNA (miRNA) biology has advanced from the initial discovery of their regulatory functions to the finding of abnormal activity in leukaemias, and then to a comprehensive understanding of the roles of miRNAs in both normal physiology and most diseases, with cancer being extensively studied. miRNA dysregulation contributes to tumorigenesis, with certain miRNAs acting as either tumour suppressors or oncogenic factors in a context-dependent manner. A…

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22 Antileukemic therapies for older adults with AML ineligible for conventional therapy: systematic review & meta-analysis

推荐理由: 该研究为2025年ASH指南更新提供了关键证据，直接针对老年AML患者这一治疗困境群体，对血液科临床实践具有重要指导价值。

摘要: Older adults with newly diagnosed acute myeloid leukemia (AML) are often ineligible for conventional “7+3” induction chemotherapy. Despite recent drug approvals, treatment outcomes remain poor in this population. We conducted an updated systematic review to inform the 2025 American Society of Hematology (ASH) AML update guidelines in older adults. This review compared the efficacy and safety of low-dose cytarabine (LDAC), azacitidine (AZA), 5- and 10-day decitabine (DEC), and gemtuzumab…

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23 Global access to commercial CAR T cells: A cross-sectional study of health technology assessment in the G20

推荐理由: 该文章聚焦CAR-T疗法在现实推广中的核心瓶颈——成本与支付政策，对血液肿瘤临床治疗和卫生政策制定具有双重借鉴意义。

摘要: Chimeric antigen receptor T-cells (CAR T) are a major treatment advance for many patients with haematological malignancies, especially those with disease that has relapsed or is refractory to chemotherapy. However, currently approved commercial CAR T-cells require highly specialised manufacturing processes that contribute to high costs and limit their widespread use. In many countries, positive reimbursement recommendations by health technology assessment (HTA) bodies enable patient access to…

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24 PU.1-Activated Genomic Regions Define Low-risk MDS Subsets Characterized by Immune Dysregulation and Disease Progression

推荐理由: 该研究通过表观基因组与转录组整合分析，前瞻性地揭示了MDS向急性白血病转化的调控机制，对理解疾病进展的生物学基础及开发预后标志物具有较高参考价值。

摘要: Myelodysplastic syndromes (MDS) are heterogeneous myeloid neoplasms with an increased risk of progression to secondary acute myeloid leukemia (sAML). This study investigates the genomic correlates of disease progression in MDS by profiling active genomic regulatory regions and their transcriptional impact through H3K27ac ChIP-seq and RNA-seq analysis on CD34+ bone marrow progenitors cells isolated from a prospective cohort of 86 and 357 patients, respectively. Our analysis revealed distinct…

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25 Single-Cell Profiling of ANKRD26 Thrombocytopenia Reveals Progenitor Expansion and Polyploid Apoptosis via JUNB-p21

推荐理由: 该研究通过创新的交叉验证方法，深入解析了ANKRD26相关血小板减少症的巨核细胞缺陷机制，为罕见血液病的病理研究和临床诊断提供了重要的实验依据与方向借鉴。

摘要: ANKRD26-related thrombocytopenia (THC2) is a rare inherited platelet disorder caused by germline variants in the 5′ untranslated region (UTR) of ANKRD26. While prior studies using in vitro models or isolated case reports have suggested impaired megakaryopoiesis as a central mechanism, detailed insights have remained elusive-primarily due to the rarity, fragility, and heterogeneity of megakaryocytes. Here, we present a comprehensive, cross-validated analysis of bone marrow samples from four…

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26 Narciclasine Alleviates Endothelial Inflammation and Atherosclerosis Initiation by Inhibiting Histone Lactylation-Mediated NF-kappaB Activation

推荐理由: 该研究揭示了糖酵解衍生的乳酸通过组蛋白乳酸化修饰调控内皮炎症的新机制，为动脉粥样硬化的表观遗传治疗提供了潜在靶点，对血管炎症相关血液疾病研究具有重要借鉴价值。

摘要: Glycolysis-derived lactate serves as a substrate for lysine lactylation, an epigenetic modification playing critical transcriptional regulatory roles in inflammatory diseases. Endothelial inflammation, characterized by upregulated glycolysis, initiates atherosclerosis, yet the contribution of histone lactylation remains undefined. Although narciclasine exhibits anti-inflammatory and antioxidant properties, its impact on endothelial inflammation in atherosclerosis is unknown. Connectivity Map…

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27 CHAMP1 is an essential regulator for human myoblast fusion and muscle development

推荐理由: 该研究揭示了CHAMP1作为MyoD共调控因子调控肌细胞融合的新机制，其发现的非经典转录调控模式对研究造血细胞分化与融合具有重要借鉴价值。

摘要: Human skeletal muscle comprises myofibers formed by fusion of thousands of myoblasts. This process depends on tightly regulated, muscle-specific fusogens, but its genetic control remains poorly understood. Here, we identify CHAMP1 (Chromosome Alignment Maintaining Phosphoprotein 1) as essential for human myoblast fusion in vitro and in vivo. Genomic and protein-interaction assays reveal a noncanonical role for CHAMP1 as a MyoD cofactor that directly activates expression of the key muscle fusogen…

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28 CSF1R marks a subset of foetal haematopoietic multipotent progenitor cells with acute myeloid leukaemia propagation properties

推荐理由: 该研究针对KMT2A::MLLT3重排婴儿白血病这一临床难题，深入探索其细胞起源与谱系选择机制，为理解白血病发生及未来靶向治疗提供了关键理论依据，对血液肿瘤领域具有重要借鉴价值。

摘要: KMT2A-rearranged infant leukaemia is one of the most severe malignancies in infants and children, and is characterised by a very aggressive phenotype and lineage plasticity. KMT2A::MLLT3 is among the most common translocations initiating leukaemia in infants, where it can manifest with a myeloid or lymphoid leukaemia phenotype. The cell-of-origin and the mechanisms driving lineage choice in KMT2A::MLLT3+ infant leukaemia are poorly understood. In this study, we show that a subset of foetal…

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29 TidyMass2: advancing LC-MS untargeted metabolomics through metabolite origin inference and metabolic feature-based functional module analysis

推荐理由: 该文章提出的TidyMass2框架显著提升了非靶向代谢组学的数据解析能力与代谢物溯源精度，其方法学创新对血液疾病代谢机制研究具有重要的工具借鉴价值。

摘要: Untargeted metabolomics provides a direct window into biochemical activities but faces critical challenges in determining metabolite origins and interpreting unannotated metabolic features. Here, we present TidyMass2, an enhanced computational framework for Liquid Chromatography-Mass Spectrometry (LC-MS) untargeted metabolomics that addresses these limitations. TidyMass2 introduces three major innovations compared to its predecessor, TidyMass: (1) a comprehensive metabolite origin inference…

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30 DNA end configurations dictate synaptic complex formation during NHEJ-mediated end bridging

推荐理由: 该研究利用先进的单分子FRET技术揭示了DNA末端构型在非同源末端连接（NHEJ）中的关键作用，为理解血液系统恶性肿瘤（如白血病）中基因组不稳定的分子机制提供了重要的机制参考。

摘要: Synapsis, the physical bridging of two broken DNA ends, is a critical step in non-homologous DNA end joining (NHEJ), the primary pathway for repairing DNA double-strand breaks (DSBs) in vertebrates. While NHEJ synapsis has been well characterized using blunt-ended DNA, how diverse DNA end configurations influence synaptic complex formation remains much less clear. Here, using single-molecule FRET, we show that end configurations play a decisive role in synapsis pathway choice, with end pairing…

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31 Control of telomerase recruitment and end protection by independent shelterin components

推荐理由: 该研究通过开发单细胞端粒酶活性监测技术，深化了对端粒状态转换机制的理解，对研究造血干细胞衰老及血液系统退行性疾病具有重要参考价值。

摘要: Telomeres are proposed to alternate between “closed” states, in which chromosome ends are protected from DNA damage signaling and inaccessible to telomerase, and “open” states, where they become accessible for telomerase mediated elongation but less protected. Whether these states reflect distinct molecular mechanisms or mutually exclusive structural conformations remains unclear. Here, we develop a single-cell assay to monitor telomerase activity in mouse embryonic stem cells. Using this…

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32 Baicalin attenuates HIF-1alpha-driven histone lactylation and neutrophil extracellular trap (NET) formation in autoimmune uveitis

推荐理由: 该研究揭示了HIF-1α调控中性粒细胞代谢与NET形成的新机制，为血液病领域理解中性粒细胞介导的炎症反应提供了重要的病理生理学借鉴。

摘要: Uveitis is a group of vision-threatening intraocular inflammatory disorders that can affect the uveal tract, retina, optic nerve, and vitreous, leading to irreversible structural damage and vision loss. Neutrophil extracellular trap (NET) formation is a key driver of acute uveitis (AU) pathology, but the immunometabolic mechanisms governing NETosis remain unclear. Here, we identified hypoxia-inducible factor-1α (HIF-1α) as a central regulator that links neutrophil metabolism to NET formation….

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33 Somatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B cell but not T cell development and function

推荐理由: 该研究通过人类体细胞CBL功能缺失模型，揭示了CBL在B细胞发育中的关键作用，修正了小鼠模型得出的结论，对血液肿瘤和免疫缺陷相关研究具有直接借鉴价值。

摘要: The E3 ubiquitin ligase Casitas B-lineage lymphoma (CBL) promotes positive selection and antigen responses in mouse T lymphocytes by ubiquitinating ZAP70. Conversely, mouse CBL and CBL-B mutually redundantly regulate SYK ubiquitination and B cell receptor signaling. Here we studied individuals with somatically homozygous CBL loss-of-function variants in leukocytes. Human CBL is largely redundant for the development and function of human T cells. Conversely, B cell development is altered at the…

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34 Multi-omics profiling of cachexia-targeted tissues reveals a spatio-temporally coordinated response to cancer

推荐理由: 该研究采用多组学整合与时空代谢分析，方法学严谨且创新性强，其揭示的肿瘤-宿主互作机制对血液系统恶性肿瘤相关恶病质研究具有重要借鉴价值。

摘要: Cachexia is a wasting disorder associated with high morbidity and mortality in patients with cancer. Tumour-host interaction and maladaptive metabolic reprogramming are substantial, yet poorly understood, contributors to cachexia. Here we present a comprehensive overview of the spatio-temporal metabolic reprogramming during cachexia, using integrated metabolomics, RNA sequencing and ^(13)C-glucose tracing data from multiple tissues and tumours of C26 tumour-bearing male mice at different disease…

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35 Radiotherapy plus neoadjuvant and concomitant IL-13Rα2-directed immunotoxin therapy for diffuse intrinsic pontine glioma

推荐理由: 该研究虽聚焦神经肿瘤，但其基于IL-13Rα2的靶向免疫治疗策略对血液肿瘤（如CAR-T疗法开发）具有重要借鉴价值，且临床前数据扎实，值得血液领域研究者参考。

摘要: Radiotherapy (RT) is the standard-of-care for diffuse intrinsic pontine glioma (DIPG); however, it functions as a palliative treatment. Interleukin 13 receptor subunit alpha 2 (IL-13Rα2) is upregulated in most DIPG tumors, posing a promising therapeutic target. Immunotherapies harnessing IL-13Rα2 to selectively deliver cytotoxic payloads such as pseudomonas exotoxin A (PE) are safe in DIPG patients and efficacious in preclinical disease models. Here, we used DIPG cell lines and mouse models to…

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36 Research progress on lactylation modification in pathogenesis of osteoarthritis

推荐理由: 该文章聚焦乳酸化修饰这一新兴表观遗传机制，为代谢与疾病调控提供了创新视角，虽直接血液病关联有限，但其方法论与多组学整合思路对血液肿瘤代谢研究具有可借鉴意义。

摘要: CONCLUSION: Lactylation modification provides a novel perspective for understanding the metabolic and epigenetic mechanisms of OA and may serve as a potential biomarker and therapeutic target. Future studies combining multi-omics approaches to map the global lactylation landscape, together with small-molecule inhibitors, epigenetic editing tools, and regenerative medicine strategies, may enable precise regulation of lactylation, offering new strategies to delay or even reverse OA progression.

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37 Microbial activation of the GLP-2R mitigates gastrointestinal inflammation

推荐理由: 该研究通过微生物蛋白调控肠道菌群与免疫系统的相互作用，为炎症相关血液疾病的机制研究与干预策略提供了新颖的跨学科视角，具有启发意义。

摘要: There is an urgent need for sustainable protein sources to meet rising global nutritional demands. Here, we show that a commercially scalable microbial lysate from Methylococcus capsulatus Bath (McB), used as a dietary protein, orchestrates host-diet-microbe interactions that protect against gastrointestinal inflammation. McB administration rapidly reshapes the gut microbiota and upregulates microbial fermentation pathways, while robustly increasing peripherally induced regulatory T cells…

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38 Improved in vivo gene knockout with high specificity using multiplexed Cas12a sgRNAs

推荐理由: 该研究提出的多重sgRNA-Cas12a系统显著提高了基因敲除的效率和可靠性，为血液疾病相关基因功能研究和遗传筛选提供了更优的技术方案，具有较高的方法学借鉴价值。

摘要: CRISPR nuclease-mediated gene knock-out is limited by suboptimal sgRNAs, inaccessible target sites, and undesired repair outcomes. Here, we present a Cas12a-based system in Drosophila that targets each gene with four sgRNAs to overcome these limitations. Multiplexed sgRNAs act through redundancy and synergism, frequently creating deletions between target sites and increasing the fraction of loss-of-function mutations. We show that multiplexed gene targeting is well tolerated and does not cause…

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39 Reducibility of higher-order networks from dynamics

推荐理由: 该文章提出的高阶网络定量方法为研究血液系统中细胞群体相互作用、信号通路网络或凝血级联反应等复杂机制提供了新的建模思路，对血液病复杂病理的网络分析具有潜在借鉴价值。

摘要: Empirical complex systems can be characterized not only by pairwise interactions, but also by higher-order (group) interactions influencing collective phenomena, from metabolic reactions to epidemics. Nevertheless, higher-order networks’ apparent superior descriptive power-compared to classical pairwise networks-comes with a much increased model complexity and computational cost, challenging their application. Consequently, it is of paramount importance to establish a quantitative method to…

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40 Bioadaptive Ni single atoms unlock high rate microbial electrosynthesis of isopropanol from CO<sub>2</sub>

推荐理由: 该研究展示了单原子催化剂与合成生物学结合的前沿技术，其微生物代谢改造策略对血液病相关生物合成研究具有方法学借鉴价值。

摘要: Hybrid systems that integrate electrochemical CO(2) reduction with microbial upgrading offer a viable route to high value organic compounds from CO(2) at ambient conditions. However, electrocatalyst deactivation in microbial growth media remains a key barrier, limiting efficiency and increasing cost. Here we show that a bioadaptive single-atom nickel catalyst (Ni SAC), coupled with genetically engineered Clostridium ljungdahlii, enables robust electrosynthesis of isopropanol (IPA) from CO(2) via…

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41 HEXIM1 inter-monomer autoinhibition governs 7SK RNA binding specificity and P-TEFb inactivation

推荐理由: 该研究通过解析Hexim蛋白与7SK RNP调控P-TEFb的分子机制，为血液肿瘤（如白血病）中转录异常调控提供了关键机理参考，其结构生物学方法对血液疾病靶点研究具有直接借鉴价值。

摘要: Hexim proteins are key RNA-dependent regulators of eukaryotic transcription through 7SK-dependent sequestration and inactivation of the kinase P-TEFb (Cdk9-CyclinT1/2) in the 7SK RNP. P-TEFb activity drives release of RNA polymerase II from promoter-proximal pausing for eukaryotic and HIV-1 transcription. The molecular mechanism by which 7SK binding overcomes an intrinsic Hexim autoinhibition for subsequent P-TEFb inactivation has remained unresolved. Here, using NMR and biophysical methods we…

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42 A high-throughput, flow cytometry approach to measure phase behavior and exchange in biomolecular condensates

推荐理由: 该文章首次将流式细胞术应用于生物分子凝聚体单液滴分析，方法创新且通量高，对研究血液肿瘤相关蛋白NPM1的相分离行为具有直接参考价值。

摘要: Biomolecular condensates are essential for cellular organization, yet their formation dynamics and molecular content exchange properties remain poorly understood. Here we show that flow cytometry provides a high-throughput, solution-based platform for analyzing condensate behavior at the single-droplet level. Using self-interacting NPM1 condensates as a model, we demonstrate that this approach quantifies phase behavior across protein and salt conditions, measures the partitioning of diverse…

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43 Single-cell screens identify ADAM12 as a fibroblast checkpoint impeding anti-tumor immunity

推荐理由: 该研究通过创新的CRISPRi/a-Perturb-seq筛选系统解析了癌症相关成纤维细胞的异质性调控机制，其前沿的系统性筛选策略对血液肿瘤微环境研究具有重要方法论借鉴价值。

摘要: Clinical trials targeting cancer-associated fibroblasts (CAFs)-crucial pro-tumoral factors in cancer-have almost all failed. This may be ascribed to their intrinsic functional plasticity and the opaque regulatory circuits underlying their heterogeneous phenotypes within tumors. We address these by developing a systematic screening approach for patient-derived fibroblasts using complementary CRISPR interference (CRISPRi) and activation (CRISPRa)-based Perturb-seq. An anti-tumoral interferon…

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44 A context-augmented large language model for accurate precision oncology medicine recommendations

推荐理由: 该研究通过检索增强生成技术提升了大型语言模型在精准肿瘤治疗中的临床实用性，为血液肿瘤等专科领域的AI辅助决策提供了可借鉴的技术框架。

摘要: The rapid expansion of molecularly informed therapies in oncology, coupled with evolving regulatory food and drug administration (FDA) approvals, poses a challenge for oncologists seeking to integrate precision oncology medicine into patient care. Large language models (LLMs) have clinical potential, but their reliance on general knowledge limits their ability to provide up-to-date and niche treatment recommendations. Here, we developed a retrieval-augmented generation (RAG)-LLM workflow using…

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45 An oral, liver-restricted LXR inverse agonist for dyslipidemia: preclinical development and phase 1 trial

推荐理由: 该文章深入探讨了LXR抑制在调节脂质代谢中的多靶点机制，对研究动脉粥样硬化等与血脂异常相关的血液系统病理变化具有重要的机制借鉴价值。

摘要: Despite advances in lipid-lowering treatment, atherosclerotic cardiovascular disease remains the leading cause of mortality, underscoring the need to address residual risk. Targeting both the synthesis and clearance of triglyceride (TG)-rich lipoproteins is a promising approach. Liver X receptor (LXR) repression can reduce plasma TG and cholesterol and improve insulin sensitivity by suppressing de novo lipogenesis and intestinal lipid absorption and enhancing clearance of TG-rich lipoproteins,…

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46 Lactate derived from cancer-associated fibroblasts promotes alternative splicing and castration resistance in prostate cancer

推荐理由: 该研究揭示了肿瘤微环境中乳酸代谢的新来源与信号机制，为理解低糖酵解肿瘤的进展提供了新视角，其基质-肿瘤代谢互作模式对血液肿瘤微环境研究具有重要借鉴价值。

摘要: Lactate in the tumor microenvironment (TME) is typically generated by cells exhibiting high glycolytic flux, exemplified by tumor cells. However, in glycolysis-low malignancies such as prostate cancer, stroma-derived lactate may drive noncanonical signaling and functions that remain unclear. Here, we identified APCDD1^(+) cancer-associated fibroblasts (CAFs) as a distinct stromal population that secretes lactate into the TME in response to androgen deprivation therapy (ADT). Lactate uptake by…

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47 Baicalin attenuates HIF-1alpha-driven histone lactylation and neutrophil extracellular trap (NET) formation in autoimmune uveitis

推荐理由: 该研究揭示了HIF-1α调控中性粒细胞代谢与NETosis的新机制，为理解NET相关血液疾病（如中性粒细胞功能障碍）提供了重要理论借鉴。

摘要: Uveitis is a group of vision-threatening intraocular inflammatory disorders that can affect the uveal tract, retina, optic nerve, and vitreous, leading to irreversible structural damage and vision loss. Neutrophil extracellular trap (NET) formation is a key driver of acute uveitis (AU) pathology, but the immunometabolic mechanisms governing NETosis remain unclear. Here, we identified hypoxia-inducible factor-1α (HIF-1α) as a central regulator that links neutrophil metabolism to NET formation….

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48 Lactate derived from cancer-associated fibroblasts promotes alternative splicing and castration resistance in prostate cancer

推荐理由: 该研究创新性地揭示了前列腺癌肿瘤微环境中CAFs分泌乳酸的非经典信号机制，为理解代谢异质性肿瘤的基质-肿瘤细胞互作提供了新视角，对研究血液肿瘤

摘要: Lactate in the tumor microenvironment (TME) is typically generated by cells exhibiting high glycolytic flux, exemplified by tumor cells. However, in glycolysis-low malignancies such as prostate cancer, stroma-derived lactate may drive noncanonical signaling and functions that remain unclear. Here, we identified APCDD1^(+) cancer-associated fibroblasts (CAFs) as a distinct stromal population that secretes lactate into the TME in response to androgen deprivation therapy (ADT). Lactate uptake by…

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49 Environmental enrichment and physical exercise prevent stress-induced social avoidance and blood-brain barrier alterations via Fgf2

推荐理由: 该研究创新性地揭示了环境干预通过保护血脑屏障紧密连接蛋白Claudin-5来缓解应激行为的机制，其血管内皮研究范式对血液病领域（如血管完整性相关疾病）具有重要借鉴价值。

摘要: Chronic stress promotes blood-brain barrier (BBB) integrity loss leading to passage of inflammatory mediators in mood-regulating brain areas and establishment of depressive behaviors. Conversely, neurovascular adaptations favoring stress resilience and preventive strategies to promote them are undetermined. We report that environmental enrichment dampens stress-induced loss of endothelial tight junction Claudin-5 (Cldn5) along with anxiety- and depression-like behaviors in male mice via an…

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50 Optimal murine CD4<sup>+</sup> T cell priming by mRNA-lipid nanoparticle vaccines requires endogenous antigen processing

推荐理由: 该研究揭示了mRNA疫苗通过细胞内抗原表达激活CD4⁺ T细胞的新机制，挑战了经典MHC II类抗原呈递认知，对疫苗免疫学及肿瘤免疫治疗研究具有重要启发价值。

摘要: Lipid nanoparticle (LNP)-encapsulated nucleoside-modified mRNA vaccines elicit robust CD4^(+) T cell responses, yet the mechanisms underlying this T cell priming remain unknown. Antigens presented to CD4^(+) T cells on major histocompatibility complex class II (MHC II) are traditionally acquired by antigen presenting cells (APCs) from extracellular sources. Here we show that vaccine-specific CD4^(+) T cell responses instead rely on antigen directly expressed within APCs, without extracellular…

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51 NALIRIFOX versus gemcitabine plus nab-paclitaxel in Chinese patients with advanced pancreatic adenocarcinoma: a randomized, open-label phase II trial

推荐理由: 该研究针对晚期胰腺癌这一难治性肿瘤，对比了两种重要化疗方案的疗效与

摘要: In this phase 2 study (NCT05047991), patients with unresectable metastatic pancreatic adenocarcinoma were randomized to receive NALIRIFOX (liposomal irinotecan, 5-FU, leucovorin, and oxaliplatin) or gemcitabine plus nab-paclitaxel. The primary endpoint was progression free survival (PFS). Secondary endpoints included other efficacy outcomes (overall survival, objective response rate, disease control rate, and duration of response), as well as safety, pharmacokinetic parameters, and evaluation of…

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52 Narciclasine Alleviates Endothelial Inflammation and Atherosclerosis Initiation by Inhibiting Histone Lactylation-Mediated NF-kappaB Activation

推荐理由: 该研究揭示了乳酸化修饰在动脉粥样硬化内皮炎症中的新机制，为血液系统炎症性疾病及血管并发症提供了潜在的表观遗传治疗靶点，对血液病代谢微环境研究具有重要借鉴意义。

摘要: Glycolysis-derived lactate serves as a substrate for lysine lactylation, an epigenetic modification playing critical transcriptional regulatory roles in inflammatory diseases. Endothelial inflammation, characterized by upregulated glycolysis, initiates atherosclerosis, yet the contribution of histone lactylation remains undefined. Although narciclasine exhibits anti-inflammatory and antioxidant properties, its impact on endothelial inflammation in atherosclerosis is unknown. Connectivity Map…

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53 Adipocytic sclerostin loop3-LRP4 interaction required by sclerostin to impair whole-body lipid and glucose metabolism

推荐理由: 该研究揭示了硬化素不同结构域（loop2与loop3）在骨代谢与心血管保护作用中的功能分离，为靶向治疗设计提供了新思路，对研究骨髓微环境及代谢相关血液疾病具有潜在借鉴价值。

摘要: Sclerostin, which has three loops, inhibits bone formation and impairs whole-body lipid and glucose metabolism. The marketed therapeutic sclerostin antibody for postmenopausal osteoporosis (POP) mainly targeting loop2 promotes bone formation and improves whole-body lipid and glucose metabolism. However, FDA/EMA warns of its cardiovascular risk. We previously demonstrate that sclerostin loop3 contributes to the inhibitory effect of sclerostin on bone formation but not its cardioprotective effect….

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54 Spin-state engineering of single titanium adsorbates on ultrathin magnesium oxide

推荐理由: 该文章在单原子尺度上操纵与探测自旋量子态的方法学极具创新性和技术严谨性，虽与血液疾病无直接关联，但其高精度表征思路对血液学中

摘要: Single atomic adsorbates on ultrathin insulating films provide a promising route towards building bottom-up quantum architectures based on atomically identical yet individually addressable spin qubits on solid surfaces. A key challenge in engineering quantum-coherent spin nanostructures lies in understanding and controlling the spin state of individual adsorbates. In this work, we investigate single titanium (Ti) atoms adsorbed on MgO/Ag(100) surfaces using a combined scanning tunneling…

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55 Cord blood DNA methylation and cell type composition are not significantly associated with severe preeclampsia, after cell type and clinical covariate adjustment

推荐理由: 该研究是一篇重要的方法学警示文章，它揭示了表观遗传学研究中因忽略混杂因素而可能产生假阳性结论的普遍问题，对于提升未来血液或发育相关表观基因组研究的严谨性具有关键的借鉴价值。

摘要: CONCLUSIONS: In summary, our study shows that the previously reported differentially methylated patterns in cord blood are actually artifacts due to not properly adjusting for cell type heterogeneity, gestational age, and clinical covariates. Severe preeclampsia is not associated with statistically significant DNA methylation changes but changes in cell proportion. This finding alerts to the scientific rigor needed in EWAS.

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56 Time to coronary angiography and revascularization in 575,247 patients with STEMI from 2012 to 2023: a retrospective population-based cohort study

推荐理由: 该研究虽聚焦心血管急症，但其对再灌注时间窗的分析、延迟因素探讨及长期死亡率数据，对血液科研究血栓性疾病的治疗时效性与预后评估具有重要借鉴意义。

摘要: BACKGROUND: Rapid primary percutaneous coronary intervention (PPCI) in patients with ST-elevation myocardial infarction (STEMI) reduces in-hospital and long-term mortality. This study analyzes time intervals to PPCI in STEMI, risk factors for delay of PPCI, and in-hospital mortality from 2012 to 2023.

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57 Psychotropic medications and their interactions with subcortical brain volume in bipolar disorder: An ENIGMA mega-analysis

推荐理由: 该研究采用大规模荟萃分析并创新性地依据用药状态分类，方法学严谨，对探讨药物影响脑结构的模式具有参考价值，其研究思路可借鉴于血液疾病治疗与影像学的交叉分析。

摘要: MRI studies in bipolar disorder (BD) have yielded inconsistent findings, partly due to the varied use of psychotropic medications. This study utilised a mega-analysis approach, accounting for concurrent medication status (syndrome-based and Neuroscience-based Nomenclature (NbN) classifications), in order to assess the association of medication status with subcortical brain volumes in BD. Data from 2,664 BD patients and 4,065 controls (CN) were pooled from 34 research groups as part of the ENIGMA…

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58 Salvianolic acid C protects against sepsis-associated acute kidney injury through promotion of PGC1α-mediated renal gluconeogenesis

推荐理由: 该研究揭示了代谢重编程（PGC1α-糖异生）与表观遗传修饰（组蛋白乳酸化）之间的新联系，其机制思路对研究血液

摘要: CONCLUSION: Our findings uncover a novel PGC1α-gluconeogenesis-histone lactylation axis through which SAC protects against SA-AKI, thereby highlighting metabolic regulation as a promising therapeutic strategy for SA-AKI.

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59 Lactylation-driven nuclear RIG-I promoted by lactate transporter inhibitor suppresses DNA damage repair through inhibiting PARP1 activity

推荐理由: 该研究揭示了RIG-I乳酸化修饰及其核转位的新机制，为理解先天免疫与肿瘤代谢交叉调控提供了新视角，对血液系统肿瘤的免疫治疗研究具有潜在借鉴价值。

摘要: RIG-I (DDX58) is typically localized in the cytoplasm and activates innate immunity. However, the mechanisms governing its nuclear translocation and functions remain incompletely understood. Here, we discover that RIG-I undergoes lactylation, which is mediated by the acetyltransferase PCAF. Treatment with the lactate transporter inhibitor syrosingopine blocks the efflux of lactate from cancer cells, increasing intracellular lactate concentration, promoting RIG-I lactylation, and enhancing the…

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60 Lactylation-driven nuclear RIG-I promoted by lactate transporter inhibitor suppresses DNA damage repair through inhibiting PARP1 activity

推荐理由: 该研究首次揭示了RIG-I的乳酸化修饰及其核转位机制，为理解先天免疫与肿瘤代谢交叉调控提供了新视角，对血液系统肿瘤（如淋巴瘤、白血病）的免疫代谢研究具有

摘要: RIG-I (DDX58) is typically localized in the cytoplasm and activates innate immunity. However, the mechanisms governing its nuclear translocation and functions remain incompletely understood. Here, we discover that RIG-I undergoes lactylation, which is mediated by the acetyltransferase PCAF. Treatment with the lactate transporter inhibitor syrosingopine blocks the efflux of lactate from cancer cells, increasing intracellular lactate concentration, promoting RIG-I lactylation, and enhancing the…

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61 Dimensionality-dependent electronic and vibrational dynamics in low-dimensional organic-inorganic tin halides

推荐理由: 该研究通过维度调控揭示了激子-声子耦合的物理机制，对开发新型光电材料具有重要启示，其研究范式可借鉴于血液检测中荧光探针的优化设计。

摘要: Photo-induced dynamics of electronic processes are driven by the coupling between electronic and nuclear degrees of freedom. Here, we construct one- and two-dimensional organic-inorganic tin halides to investigate how dimensionality controls exciton-phonon coupling and exciton self-trapping. The results show that a one-dimensional system has strong exciton-phonon coupling leading to excitation-independent self-trapped exciton emission, whereas a two-dimensional system exhibits over ten times…

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62 Thiazole-conjugated covalent organic framework membranes enable ultraselective molecular desalination under strongly acidic conditions

推荐理由: 该文章提出了一种通用的一步法合成高稳定性共价有机框架膜的策略，材料创新性强、方法具有可扩展性；虽不直接针对血液疾病，但其高精度分子分离技术可能为血液净化或血液成分分离提供潜在的膜材料借鉴思路。

摘要: Membrane nanofiltration provides a sustainable and energy-efficient platform for precise molecular separation. However, highly permselective and chemically stable membrane materials capable of operating under harsh conditions are currently lacking. Here we report a generalizable monomer-solvent dual engineering strategy that enables the one-step synthesis of chemically robust thiazole-linked polycrystalline covalent organic framework (COF) membranes via scalable interfacial polymerization under…

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63 A physics-informed graph neural network conserving linear and angular momentum for dynamical systems

推荐理由: 该研究提出的物理信息图神经网络方法在可解释性和泛化性上具有显著创新，其跨学科建模思路可为血液动力学或多细胞相互作用模拟提供方法论借鉴。

摘要: Accurate, interpretable, and real-time modeling of multi-body dynamical systems is essential for predicting behaviors and inferring physical properties in natural and engineered environments. Traditional physics-based models face scalability challenges and are computationally demanding, while data-driven approaches like Graph neural networks (GNNs) often lack physical consistency, interpretability, and generalization. In this paper, we propose DYNAMI-CAL GRAPHNET, a Physics-Informed Graph Neural…

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64 Genetic insights and mechanistic parallels in gestational diabetes mellitus and type 2 diabetes

推荐理由: 该研究在遗传关联分析方法上严谨创新，虽非直接针对血液疾病，但其跨组织转录组分析策略可为血液病遗传机制

摘要: Gestational diabetes mellitus (GDM) has a high heritability and frequently co-occurs with type 2 diabetes (T2D), indicating shared genetic mechanisms. Firstly, we employed RHOGE to ascertain the genetic correlation and putative causal directions between GDM and T2D. Subsequently, the Genotype-Tissue Expression Project v8 eQTls files and the FinnGen R11 dataset were employed to conduct cross-tissue transcriptome association studies, Functional Summary-based Imputation in single tissues, and Gene…

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65 A self-breathing electrode enabled by interface regulation and gradient wettability engineering for industrial H<sub>2</sub>O<sub>2</sub> electrosynthesis

推荐理由: 该研究通过创新的非熔融颗粒堆积界面与三维重构技术，揭示了电化学产过氧化氢中三相界面形成机制，其方法学与界面设计思路对血液病相关氧化应激研究或体外消毒技术具有潜在借鉴价值。

摘要: High-performance gas diffusion electrodes (GDEs) are essential for electrochemical H(2)O(2) production, yet conventional catalyst layers (CLs) suffer from PTFE-fused encapsulation and disordered pores that create mass-transport bottlenecks and suppress three-phase interface (TPI) formation. Here, we introduce a non-fused particulate-packed catalyst/binder interface and elucidate the mechanisms governing TPI formation through 3D reconstruction and mesoscale LBM analyses. Guided by these insights,…

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66 Multiomic integration reveals tumoral heterogeneity of lipid dependence within lethal group 3 medulloblastoma

推荐理由: 该研究采用多组学整合方法揭示了髓母细胞瘤的代谢异质性，其大规模数据整合策略和高通量技术对血液肿瘤的多组学研究具有重要的方法论借鉴价值。

摘要: Medulloblastoma, the most common malignant brain tumor of childhood, exhibits significant biological complexity that demands deeper exploration. Here, we present a large multiomics dataset integrating data from 384 primary medulloblastoma patient samples across five omic layers: CpG methylome, transcriptome, proteome, phosphoproteome, and metabolome, paired with associated clinical metadata. Data integration revealed intertumoral heterogeneity of lipid metabolism across proteomic subtypes….

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67 Germline variants in ATM, BRCA2, other cancer predisposition and novel candidate genes are implicated in glioma risk in adult glioma patients with a familial or personal history of tumors

推荐理由: 该研究采用全外显子测序和双重分析策略探究神经胶质瘤的遗传易感性，方法学严谨，数据可靠，对血液病领域遗传倾向研究具有方法论借鉴价值。

摘要: Familial occurrence of gliomas has been reported in around 5% of patients. Studies on the genetic landscape of glioma predisposition are scarce. Here, leukocyte DNA of 213 adult glioma patients with a familial and/or personal tumor history from 206 families was subjected to whole-exome sequencing. Germline variants (GVs) were analyzed using two approaches: (1) GVs in 164 established cancer predisposition genes (CPGs) or suspected glioma risk genes were extracted and classified; (2) the…

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68 Remodeling of <em>XIST</em> regulatory landscape during primate evolution

推荐理由: 该研究从进化角度揭示XIST调控的快速演变，为理解X染色体失活相关血液疾病（如某些X连锁疾病）的分子基础提供了新的演化生物学视角，对血液病机制研究具有启发意义。

摘要: Unraveling how gene regulations are remodeled during evolution is central to understanding how biological processes evolve. We explored this question in the frame of X-chromosome inactivation, a process under strong selective constraint, governed by the XIST lncRNA and its cis-regulators. Using functional approaches on closely related primate species, we show that XIST regulation has uniquely diverged over a short evolutionary timescale. In human and marmoset embryonic stem cells (ESCs), the JPX…

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69 Breaking the oxo-wall for Co(IV)-oxo species and their nanoconfined catalytic performance within Ce-Co lamellar membrane

推荐理由: 该研究在突破“氧墙”限制方面提供了创新的理论框架和材料设计思路，对涉及金属氧化态调控的交叉学科（如仿生催化）具有较高的方法论借鉴价值。

摘要: Co(IV) = O-mediated Fenton-like processes show great potential for water remediation but are fundamentally limited by the “oxo-wall” effect, which imposes prohibitive activation energies for Co(IV) = O bond formation and stabilization. Herein, by unifying thermodynamic analysis with the “oxo-wall” constraint mechanism, we establish the comprehensive theoretical framework for Co(IV) = O-dominated non-radical Fenton-like oxidation pathways. We design a Ce-Co tetra-(4-carboxyphenyl) porphyrin…

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70 An artificial cell capable of signal transduction mediated by ADRB2 for the regulation of glycogenolysis

推荐理由: 该研究在人工细胞中成功重构GPCR信号通路，方法前沿且具有高度创新性，为血液病中相关受体信号转导机制的研究提供了有价值的体外模型参考。

摘要: Bottom-up construction of artificial cells helps elucidate the working mechanism of cells. Signal transduction from extracellular to intracellular artificial cells is essential for autonomous artificial cells. It remains highly challenging to reconstitute G protein-coupled receptor (GPCR) signaling pathways to regulate downstream metabolism in artificial cells. Here, we reconstitute β2-adrenergic receptor, Gs subunit α and adenylate cyclase V into artificial cell membranes to enable signal…

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71 Spatiotemporally controlled drug release via a click-release system utilizing mono-alkyl-hydroxylamine and cyclooctyne chemistry

推荐理由: 该研究提出的点击释放策略在生物正交化学领域具有显著创新性，其高效可控的释放机制可为血液病靶向药物递送系统提供重要的技术借鉴。

摘要: The advancement of bioorthogonal bond-breaking chemistry requires precise spatiotemporal control over chemical reactions. In this study, we introduce a click-release strategy based on the reaction between mono-alkyl-hydroxylamine and cyclooctyne (COT), enabling rapid and nearly complete payload release. We demonstrate that mono-alkyl-hydroxylamine and hydroxylamine react with COT to form nitrone and oxime, respectively, facilitating a versatile and efficient release mechanism. By conjugating…

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72 Systems acclimation to osmotic stress in zygnematophyte cells

推荐理由: 该研究采用多组学方法系统解析藻类应对渗透压胁迫的机制，其前沿的实验设计与分析思路可为血液病领域研究细胞应激反应提供方法学借鉴。

摘要: Zygnematophytes are the closest algal relatives of land plants. They hold key information to infer how the earliest land plants overcame the barrage of terrestrial stressors, the prime of which is osmotic stress. Here, we apply two osmotic stressors on a unicellular and a multicellular representative of zygnematophytes and study their responses over a 25-hour time course, generating 130, 60, and 30 transcriptomic, proteomic, and metabolomic samples combined with photophysiology, sugar analysis,…

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73 Multiscale imaging on proton pump-driven acidity for assessing tumor progression and metastasis

推荐理由: 该研究聚焦于肿瘤酸性微环境的关键调控因子ATP6V0C，其揭示的质子转运机制不仅对实体瘤诊疗有创新价值，也可能为白血病等血液肿瘤的微环境研究提供新的分子靶点和可借鉴的思路。

摘要: An acidic tumor microenvironment (TME), a hallmark of cancer progression, promotes tumor growth, invasion, and metastasis. Detecting and targeting tumor acidity have emerged as key frontiers in early cancer diagnosis and treatment. However, current approaches lack sensitive and specific methods to visualize and quantify tumor acidity in vivo across different stages of tumor development. Here we show that ATP6V0C, a subunit of the V-ATPase responsible for proton transmembrane transport, is a…

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74 Polymerase mutations underlie early adaptation of H5N1 influenza virus to dairy cattle and other mammals

推荐理由: 该研究揭示了H5N1病毒在哺乳动物宿主中快速进化的分子机制，虽非直接血液病研究，但其病毒适应性研究方法对血液系统感染病原体研究具有重要借鉴意义。

摘要: In 2024, an unprecedented outbreak of H5N1 high pathogenicity avian influenza was detected in dairy cattle in the USA resulting in spillbacks into poultry, wild birds and other mammals including humans. Here, we present molecular and virological evidence that the cattle B3.13 genotype H5N1 viruses rapidly accumulated adaptations in polymerase genes that enabled better replication in bovine cells and tissues, as well as cells of other mammals including humans. We find evidence of several…

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75 Tissue-specific and sex-biased glycoproteomic landscape of Schistosoma mansoni

推荐理由: 该研究在寄生虫糖基化组学方法学上有显著创新，整合多组学技术揭示了血吸虫免疫逃逸的分子机制，虽非直接研究血液疾病，但对理解血液中寄生生物的宿主互作及抗原设计具有间接参考价值。

摘要: Schistosomiasis is a major global health burden, with praziquantel as the sole treatment. Its inability to prevent reinfection highlights the need for new interventions such as vaccines. Protein glycosylation is essential for parasite biology and immune evasion, yet remains poorly characterized in schistosomes. Here, we conduct N- and O-glycoproteomic and intact glycopeptides analyses of adult male and female Schistosoma mansoni, integrating single-cell transcriptomics. We uncover…

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76 Estimating COVID-19 incidence and prevalence using lateral flow tests in England and Scotland, 2023-2024

推荐理由: 该研究提供了大规模社区感染监测的方法学范例，其严谨的流行病学设计对血液病流行病学研究具有可借鉴的参考价值。

摘要: SARS-CoV-2 continues to cause substantial morbidity and mortality, particularly in winter. During the SARS-CoV-2 pandemic, community prevalence surveys provided detailed monitoring of infection levels. The Winter Coronavirus (COVID-19) Infection Survey (WCIS), conducted in England and Scotland from the 14^(th) November 2023 to the 7^(th) March 2024, enabled the UK Health Security Agency to publish fortnightly estimates of community infection levels in England and Scotland. Unlike previous…

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77 Salvianolic acid C protects against sepsis-associated acute kidney injury through promotion of PGC1α-mediated renal gluconeogenesis

推荐理由: 该研究揭示了代谢重编程与表观修饰（乳酸化）在危重症并发症中的新机制，为血液病领域研究代谢-免疫-表观调控网络提供了可借鉴的思路。

摘要: CONCLUSION: Our findings uncover a novel PGC1α-gluconeogenesis-histone lactylation axis through which SAC protects against SA-AKI, thereby highlighting metabolic regulation as a promising therapeutic strategy for SA-AKI.

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78 Safety and efficacy of oral microbiome therapy for the treatment of recurrent <em>Clostridioides difficile</em> infection: a systematic review and meta-analysis of randomized controlled trials

推荐理由: 该研究虽主要针对胃肠道感染，但其微生物群调节疗法对血液病患者（特别是免疫抑制或造血干细胞移植后合并艰难梭菌感染）的治疗策略有重要借鉴意义，且结论具有临床实用性。

摘要: CONCLUSION: OMT seems to reduce CDI recurrence at week 8 compared with placebo while demonstrating a comparable safety profile, supporting its role as an effective, well-tolerated therapy for recurrent CDI. New studies are necessary to confirm these findings.

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79 Tho1 and MOS11 promote nucleic acid double-strand unwinding by facilitating DEAD-box helicase oligomerization

推荐理由: 该研究揭示了RNA解旋酶活性调控的新机制，对理解DDX39B/UAP56在血液系统疾病（如骨髓增生异常综合征）中的突变影响具有潜在借鉴价值。

摘要: DEAD-box helicases are essential for gene expression and RNA metabolism. However, the mechanisms regulating their activity remain largely elusive. The DEAD-box helicase DDX39B/UAP56 forms a 2:1 complex with the C-terminal domain (CTD) of RNA-binding protein Tho1, but the functional relevance of this interaction is still elusive. Here, we show that the Tho1-CTD stimulates the helicase activity of Sub2, the yeast homologue of DDX39B/UAP56, by acting as a rigid scaffold that promotes Sub2…

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80 Higher sun exposure is associated with a reduced risk of primary biliary cholangitis

推荐理由: 该研究首次揭示了日照与原发性胆汁性胆管炎（PBC）风险之间的负相关关系，为理解环境因素在自身免疫性疾病中的作用提供了新的重要线索，对血液免疫相关领域的研究具有启发和借鉴价值。

摘要: CONCLUSION: We have demonstrated for the first time that higher sun exposure and UV load, particularly in summer, were consistently associated with a reduced risk of developing PBC.

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81 Current and Emerging Therapies for C3 Glomerulopathy and Primary (Idiopathic) Immune Complex Membranoproliferative Glomerulonephritis

推荐理由: 该文章聚焦补体系统失调在肾脏疾病中的作用，而补体异常与阵发性睡眠性血红蛋白尿（PNH）、非典型溶血尿毒综合征（aHUS）等多种血液病密切相关，其机制研究和治疗策略对血液病领域有重要的交叉借鉴价值。

摘要: C3 glomerulopathy (C3G) and primary (idiopathic) immune complex membranoproliferative glomerulonephritis (IC-MPGN) are rare kidney diseases characterized by dysregulation of the complement system and progressive deposition of C3 and its breakdown products in the glomeruli, ultimately leading to kidney failure in up to 50% of patients within 10 years. Until recently, standard approaches to treatment included supportive measures common to many kidney diseases and immunosuppression to mitigate…

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82 Prediction of interactions between the SARS-CoV-2 ORF3a protein and small-molecule ligands using the ANDSystem cognitive platform, graph neural networks, and molecular modeling

推荐理由: 该文章提出的基于异质图与图神经网络的分子相互作用预测方法具有较高的方法学创新性与技术可借鉴性，虽未直接聚焦血液疾病，但其网络重建与关系预测框架可为血液学领域的基因-疾病网络研究提供重要的方法论参考。

摘要: In recent years, artificial intelligence methods based on the analysis of heterogeneous graphs of biomedical networks have become widely used for predicting molecular interactions. In particular, graph neural networks (GNNs) effectively identify missing edges in gene networks - such as protein-protein interaction, gene-disease, drug-target, and other networks - thereby enabling the prediction of new biological relationships. To reconstruct gene networks, cognitive systems for automatic text…

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83 iNKT cell activation exacerbates isoproterenol-induced cardiac injury through macrophage IFN-gamma-STAT1 signaling

推荐理由: 该研究聚焦免疫疗法相关心脏毒性的机制，虽非直接针对血液病，但其对iNKT细胞作用的探讨为血液肿瘤免疫治疗的安全性研究提供了可借鉴的分子通路视角。

摘要: AIMS: With the extensive application of immunotherapy in treating cancer, the immunotherapy-related cardiovascular toxicity (ITR-CVT) has gotten a rapid recognition due to its high mortality. Previously, we have found that potential cancer immunotherapies based on promoting iNKT cell activation exacerbate ISO-induced cardiac injury, but the underlying mechanism is unknown. The current study is to determine which specific cell type/s and the corresponding molecular pathways are responsible for…

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84 Research progress on lactylation modification in pathogenesis of osteoarthritis

推荐理由: 该文虽聚焦骨关节炎，但其揭示的乳酸化修饰这一新型代谢-表观遗传调控机制，为血液疾病中代谢重编程与表观遗传交叉研究提供了重要的范式借鉴与思路启发。

摘要: CONCLUSION: Lactylation modification provides a novel perspective for understanding the metabolic and epigenetic mechanisms of OA and may serve as a potential biomarker and therapeutic target. Future studies combining multi-omics approaches to map the global lactylation landscape, together with small-molecule inhibitors, epigenetic editing tools, and regenerative medicine strategies, may enable precise regulation of lactylation, offering new strategies to delay or even reverse OA progression.

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85 Classical criticality via quantum annealing

推荐理由: 该研究展示了量子退火在模拟磁性材料相变方面的独特优势，其方法学创新可为血液病中复杂细胞信号网络或异常蛋白聚集的模拟研究提供跨学科借鉴思路。

摘要: Quantum annealing provides a powerful platform for simulating magnetic materials and realizing statistical physics models, presenting a compelling alternative to classical Monte Carlo methods. We demonstrate that quantum annealers can accurately reproduce phase diagrams and simulate critical phenomena without suffering from the critical slowing down that often affects classical algorithms. To illustrate this, we study the piled-up dominoes model, which interpolates between the ferromagnetic 2D…

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86 OCT4 enhances the firing efficiency of late DNA replication origins in mouse embryonic stem cells

推荐理由: 该研究通过高精度方法绘制了胚胎干细胞复制起始区图谱，其技术路线与发现对研究造血干细胞快速增殖与基因组稳定性具有潜在借鉴价值。

摘要: DNA replication initiates at specific genomic regions known as initiation zones (IZs), which follow a defined spatiotemporal program that is partially dependent on cell type. Here, we examine the replication-initiation patterns of pluripotent mouse embryonic stem cells (mESCs), which are characterized by a very short G1 phase and rapid entry into S phase. Using EdU-seq combined with cell-cycle synchronization and Repli-seq, we identify IZs that activate during S phase in mESCs and classify them…

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87 Reducing bulky medical images via shape-texture decoupled deep neural networks

推荐理由: 该研究提出的新型医学数据压缩方法在技术创新与工程优化方面具有显著价值，虽未直接涉及血液疾病，但其高效数据处理能力可为血液学研究中的大规模数据管理提供底层技术支持。

摘要: The explosive growth of medical data poses significant challenges for storage and sharing. Current compression techniques utilizing Implicit Neural Representations (INRs) effectively strike a balance between encoding accuracy and compression ratio, yet they suffer from slow encoding speeds. By contrast, data-driven compressors encode fast but heavily rely on the training data and cannot generalize well. To develop a practical compression tool overcoming all these limitations, we introduce…

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88 Multiomic integration reveals tumoral heterogeneity of lipid dependence within lethal group 3 medulloblastoma

推荐理由: 该研究在方法学上具有高度创新性和严谨性，其多组学整合策略为肿瘤异质性研究提供了优秀范例，虽非血液病直接相关，但对从事癌症代谢或组学研究的血液学学者仍有重要参考价值。

摘要: Medulloblastoma, the most common malignant brain tumor of childhood, exhibits significant biological complexity that demands deeper exploration. Here, we present a large multiomics dataset integrating data from 384 primary medulloblastoma patient samples across five omic layers: CpG methylome, transcriptome, proteome, phosphoproteome, and metabolome, paired with associated clinical metadata. Data integration revealed intertumoral heterogeneity of lipid metabolism across proteomic subtypes….

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89 DMDGRN: A data augmentation-based multilayer directed graph convolutional network for gene regulatory network inference

推荐理由: 该研究提出的基因调控网络推断方法具有较好的创新性和应用潜力，虽然以乳腺癌为例，但其计算生物学方法对血液疾病机制研究有可借鉴的参考价值。

摘要: CONCLUSIONS: The findings demonstrate that the strategies we adopted are effective for inferring GRNs. The successful application to breast cancer data further highlights its potential of DMDGRN in uncovering disease-relevant regulatory mechanisms and identifying therapeutic targets, making it a promising tool for advancing both computational biology and translational medicine.

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90 Cascading chirality from molecule to twisted microstructures with amplified circularly polarized luminescence

推荐理由: 该文章在方法学上具有显著借鉴价值，其结合变温光谱与原位实时显微技术解析动态组装过程的研究思路，对于血液学中涉及分子自组装或动态过程（如异常蛋白聚集、细胞膜动力学）的课题，提供了先进的技术范式和跨尺度研究的创新视角。

摘要: Control of chiral assembly across length scales is desirable but challenging. Herein, we demonstrate controllable construction of microscopic chiral twists through hierarchical assembly of enantiomeric styrylpyridinium-derived glutamide amphiphiles (L/D-SPG). By combining variable-temperature spectroscopy with in-situ real-time optical microscopic observation, the dynamic chirality transfer process across spatial and length scales during assembly is elucidated. It is revealed that hydrophobic…

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91 Spatiotemporally ordered topological transformation in layered double hydroxides enables synergistic mineralization of As<sup>III</sup>/Cd<sup>2</sup>

推荐理由: 该研究在环境重金属协同去除材料上取得突破性吸附性能，虽非直接血液病研究，但砷镉毒性机制与血液系统疾病（如贫血、骨髓抑制）高度相关，其吸附机制对研究重金属致血液毒性有重要借鉴价值。

摘要: The co-contamination of arsenite (As^(III)) and cadmium (Cd^(2+)) poses a significant challenge in environmental remediation due to their divergent chemical speciation and transformation pathways. Here, a redox-active layered double oxide (MgMn-LDO) is designed, and achieves adsorption capacities of 821.7 mg g^(‒1) for As^(III) and 1895.6 mg g^(‒1) for Cd^(2+) in their coexisting system, presenting a state-of-the-art performance. Unexpectedly, the co-adsorption system not only enhances…

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92 Efficient solution-processed light-emitting diodes based on organic-inorganic hybrid antimony halides

推荐理由: 该文章在有机-无机杂化锑卤化物发光材料的设计上提出了创新的有机阳离子工程策略，对于材料科学与光电领域具有较高的参考价值，但直接与血液疾病研究的相关性较弱。

摘要: Organic-inorganic hybrid antimony halides are emerging emitters for solution-processed light-emitting diodes. However, achieving high-efficiency electroluminescence remains challenging resulting from the non-radiative recombination within emitters and inferior charge transport within device. Here, we develop an organic cation engineering to design a carbazole-functionalized triphenyl(9-ethyl-9H-carbazol-3-yl) phosphonium (TPPEtCz^(+)), which enables a (TPPEtCz)(2)Sb(2)Br(8) film with good…

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93 Reticulating node-linker-modulator chemical spaces for modular design of alkoxide-based glasses and liquids

推荐理由: 该文章在材料科学方法学上具有显著创新性和严谨性，其多技术联用的研究范式对血液学领域开发新型生物材料或药物载体具有可借鉴的跨学科参考价值。

摘要: Glasses linked by molecular building blocks are emerging materials promising for combining the processability of glasses and designability of reticular frameworks. Achieving modular chemical space construction is key to promoting structural diversity for these glasses, which requires systematically understanding their structures and properties. Herein, we combine rheological, thermal, compositional and total scattering techniques to study glasses and liquids based on titanium, zirconium and…

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94 The cotranslational cycle of the ribosome-bound Hsp70 homolog Ssb

推荐理由: 该研究通过冷冻电镜解析了核糖体结合Hsp70的结构机制，为蛋白质折叠与翻译偶联提供了重要见解，虽不直接聚焦血液疾病，但其分子机制对研究血液系统相关的蛋白质稳态失衡（如淀粉样变性、某些血液肿瘤）具有潜在参考价值。

摘要: Coupling of ribosomal translation with cotranslational protein folding is essential for cellular homeostasis. In eukaryotes, Hsp70 and its J-domain cochaperone, the heterodimeric ribosome-associated complex (RAC), are central to this process; however, mechanistic insights into the coordination of Hsp70 function with translation remain limited. Here, we present two cryo-EM structures of the ribosome-bound yeast Hsp70 Ssb, identifying Rpl25/uL23 as the ribosomal binding site and revealing its…

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95 Alcian blue-positive stromal phenotype in basal cell carcinoma is associated with progression on first-line hedgehog inhibitors

推荐理由: 该研究虽聚焦皮肤肿瘤，但其探索靶向治疗疗效预测生物标志物的方法学对血液肿瘤研究具有可借鉴性，尤其在个体化治疗和耐药机制研究方面能提供思路参考。

摘要: Basal cell carcinoma (BCC) is the most frequent malignancy in fair-skinned populations. Although curable in most cases, approximately 4% of patients develop locally advanced or metastatic disease (advBCC) requiring systemic therapy. Hedgehog pathway inhibitors (HHIs; vismodegib/sonidegib) constitute standard first-line treatment, yet individual responses vary and no histopathological biomarker predicting therapeutic outcome exists. We conducted a retrospective, multicenter analysis of 70 BCCs…

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96 4D printing through vat photopolymerization of two-stage UV-curable liquid crystal elastomers

推荐理由: 该文章在4D打印和软体驱动器材料的空间控制方面具有较高的方法学创新性，但其内容与血液疾病研究无直接关联，仅可能为未来生物相容性支架或仿生组织的材料设计提供远期的、间接的工程学思路参考。

摘要: Liquid crystal elastomers (LCEs) offer significant promise as soft actuator materials, but their potential has not yet been fully explored for 4D printing applications. Most existing studies rely on extrusion-based printing methods, which offer limited resolution and impose constraints on fabricating intricate, free-standing structures. Moreover, it remains a significant challenge to design and spatially control liquid crystal orientation within complex 3D structures to achieve desired shape…

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97 Genomic and physiological signatures of adaptation in pathogenic fungi

推荐理由: 该研究通过比较进化分析揭示了真菌从腐生向机会性致病的转变机制，虽然并非直接针对血液病，但其对致病性进化规律的阐释对理解血液系统真菌感染（如毛孢子菌血症）的病原适应性具有间接参考价值。

摘要: Emerging fungal pathogens have detrimental impacts on crops, animals, and humans. Despite the mounting threat of these emerging fungal pathogens, little is known about their transition from saprotrophic to pathogenic lifestyles. To gain insights into fungal lifestyle transitions, we study the Trichosporonales order, which includes both saprotrophic species and opportunistic human pathogens, as a system to reveal evolutionary adaptations leading to virulence in fungi. Here we use comparative…

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98 Structure of a Brochothrix thermosphacta bacteriophage reveals cell wall adsorption mechanism in Gram-positive infecting siphophages

推荐理由: 该研究在近原子分辨率下解析了感染革兰氏阳性菌的丝状噬菌体结构，为理解噬菌体侵染机制提供了关键结构信息，虽然与血液疾病无直接关联，但其方法论对研究病原微生物感染机制具有参考价值。

摘要: Siphophages possess a long, flexible, and non-contractile tail that is responsible for host recognition, cell wall perforation, and genome delivery. Although the majority of structurally characterized siphophages target Gram-negative bacteria, those infecting Gram-positive bacteria remain elusive. Moreover, structural information concerning phage infection and genome release events in Gram-positive infecting siphophages is sparse. Here, we present a near-atomic resolution structure of…

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99 Inactive ryanodine receptors sustain lysosomal availability for autophagy by promoting ER-lysosomal contact site formation

推荐理由: 该研究在细胞器互作与钙信号机制方面具有高度创新性和方法学严谨性，虽然直接血液病关联较弱，但其揭示的溶酶体功能调控机制对研究溶酶体贮积症等血液相关疾病有潜在借鉴价值。

摘要: Lysosomal and endoplasmic reticulum (ER) Ca^(2+) release mutually influence each other’s functions. Recent work revealed that ER-located ryanodine receptor(s) (RyR(s)) Ca^(2+) release channels suppress autophagosome turnover by the lysosomes. In familial Alzheimer’s disease, inhibiting RyR hyperactivity restored autophagic flux by normalizing lysosomal vacuolar H^(+)-ATPase (vATPase) levels. However, the mechanisms by which RyRs control lysosomal function and how this involves the vATPase remain…

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100 The Diagnostic Value of Confocal Laser Endomicroscopy in Brain Tumours When Performed by Blinded, Untrained Neuropathologists

推荐理由: 该研究展示了新型成像技术（CLE）在肿瘤边界识别中的应用潜力，虽聚焦脑肿瘤，但其对活体组织实时鉴别的方法学思路对血液系统肿瘤的术中或内镜诊断具有可借鉴的参考价值。

摘要: CONCLUSIONS: CLE shows potential to distinguish between neoplastic and non-neoplastic tissue, but diagnostic accuracy remains lower than with H & E-stained slides. Training in CLE image interpretation is recommended to improve diagnostic accuracy. CLE imaging may have the potential to become a valuable tool for delineating brain tumour borders.

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101 The metabolic reprogramming of lactate in the nervous system

推荐理由: 该综述系统总结了乳酸在中枢神经系统代谢重编程的分子机制与功能，虽非直接研究血液疾病，但其对代谢调控的深入探讨可为血液肿瘤或红细胞代谢相关研究提供跨学科借鉴思路。

摘要: Lactate, a critical energetic substrate and signaling molecule in the central nervous system (CNS), plays a pivotal role in maintaining neurophysiological homeostasis and driving the pathogenesis of neurodegenerative disorders through metabolic reprogramming. Herein, this review systematically summarizes recent progress in molecular mechanisms governing lactate metabolic reprogramming as well as its multiple biological functions in the central nervous system. Under physiological conditions,…

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102 The metabolic reprogramming of lactate in the nervous system

推荐理由: 该综述系统总结了乳酸代谢重编程在中枢神经系统的分子机制与功能，虽然主题并非直接聚焦血液病，但其对代谢重编程的深入探讨可为研究血液肿瘤（如白血病）的代谢异常提供可借鉴的思路与分子视角。

摘要: Lactate, a critical energetic substrate and signaling molecule in the central nervous system (CNS), plays a pivotal role in maintaining neurophysiological homeostasis and driving the pathogenesis of neurodegenerative disorders through metabolic reprogramming. Herein, this review systematically summarizes recent progress in molecular mechanisms governing lactate metabolic reprogramming as well as its multiple biological functions in the central nervous system. Under physiological conditions,…

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103 Glutamatergic projection neurons in the basal forebrain underlie learned olfactory associational valence assignments

推荐理由: 该研究在神经科学方法学上具有创新性和严谨性，尤其在活体钙成像技术应用方面有参考价值，但其内容与血液疾病直接关联较弱，主要可借鉴其研究思路与技术手段。

摘要: Sensory perception is shaped by experience, giving stimuli behavioral significance. Basal forebrain (BF) cholinergic neurons in mice, which are crucial for arousal and motivation, also regulate sensory processing. Within BF nuclei, glutamatergic (vGlut2^(BF)) neurons receive cholinergic input and modulate behaviors, but their roles in encoding sensory significance remain unclear. Using in vivo calcium imaging, we found that vGlut2^(BF) neurons initially poorly encoded odor identity. However,…

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104 Near-infrared light-driven nanomotors-based microneedles for the active therapy of bacterial infected acne

推荐理由: 该文章在纳米药物递送和微环境调控方面具有显著创新性，其不对称修饰和自供氧策略对研究缺氧相关病理机制（如骨髓微环境）有方法学借鉴价值。

摘要: Acne, caused by Cutibacterium acnes, triggers inflammatory lesions. The hypoxic biofilm microenvironment exacerbates acne progression, while inadequate hydrogen peroxide and dense biofilm barriers hinder oxygen generation and nanomedicine penetration. Here, we develop microneedles patch loaded with near infrared-driven self-oxygenating Z@P-M nanomotors for photothermal therapy of acne. Zinc peroxide nanoparticles are asymmetrically modified with polydopamine, followed by in-situ manganese…

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105 Mechanism of trans-envelope bacterial polysaccharide secretion in Class-3 outer-membrane polysaccharide export (OPX) protein systems

推荐理由: 该研究深入

摘要: Bacterial secretion of extracellular polysaccharides is essential for surface colonization, biofilm formation, and pathogenesis. In diderm bacteria, such polymers traverse the periplasm and outer membrane (OM) through outer-membrane polysaccharide export (OPX) proteins that form secretion pores. Among them, Class-3 OPX proteins are the most widespread but lack an OM-spanning pore domain, leaving their mechanisms poorly understood. Here, we characterize WzaB from Myxococcus xanthus as a model for…

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106 Real-time visualization of sub-particle reaction anisotropy via rotary-encoded scattering microscopy

推荐理由: 该文介绍的新型光学成像技术（RESM）在纳米尺度液相化学过程观测上具有显著的方法学创新，虽然不直接面向血液疾病，但其高分辨、无标记的实时成像能力对研究

摘要: Understanding nanoscale chemical transformations within liquid environments is essential for advancing catalysis, material synthesis, and nanotechnology. However, existing optical imaging techniques face significant challenges in resolve sub-particle-level dynamics due to intrinsic limitations in spatial resolution and sensitivity. Here, we introduce an optical imaging technique, Rotary-Encoded Scattering Microscopy (RESM), that achieves real-time, label-free visualization of sub-particle…

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107 Cryo-EM structure of the human COP1-DET1 ubiquitin ligase complex

推荐理由: 该文章在泛素化调控机制的结构生物学研究方面具有较高参考价值，但未明确涉及血液疾病直接关联，适合从事相关分子机制研究的血液学学者拓展基础认知。

摘要: Ubiquitin modifications regulate fundamental cellular activities by modulating protein stability and function. The ubiquitin ligase COP1, which is present across species from plants to humans, plays a crucial role in the ubiquitination of developmental transcription factors. While COP1 can function independently, it can also be incorporated into CULLIN4-RING ubiquitin ligase (CRL4) complexes through the DET1 adaptor protein. Despite its biological significance, the structural and functional…

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108 Improving erectile function in diabetic male mice by rescuing depalmitoylated FBP1 to reduce cavernosal lactate

推荐理由: 该研究揭示了乳酸代谢异常在糖尿病并发症中的新机制，虽非直接血液疾病研究，但其对代谢-血管病理机制的深入探讨可为血液肿瘤或骨髓微环境研究提供可借鉴的思路。

摘要: The high prevalence of diabetes mellitus-induced erectile dysfunction (DMED) in males and the limited efficacy of existing therapies necessitate deeper mechanistic insights. We identify pathological lactate accumulation in the corpus cavernosum as a major driver of DMED in male mice, promoting structural degeneration and smooth muscle cell loss. Mechanistically, transcriptional repression and functional impairment of fructose-1,6-bisphosphatase 1 (Fbp1), a rate-limiting gluconeogenic enzyme,…

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109 Genetic and non-genetic factors distinctly shape the variation of the immune response in cattle

推荐理由: 该研究在系统免疫学和遗传-环境互作方面方法严谨，对免疫变异的分析框架可为血液病免疫机制研究提供方法学参考，但直接关联度有限。

摘要: Increasing agricultural production while reducing the reliance on synthetic inputs such as antibiotics is an important challenge. For cattle breeding, this implies better understanding the genetics underlying meat production and the immune response. Here, we use systems immunology to investigate the genetic and environmental drivers of immune variation in Belgian White Blue male cattle, a breed historically bred for meat production. While seasonality and other non-genetic factors account for…

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110 Mixed-model and transcriptome-wide association analyses identify transcription factors and genes associated with colorectal cancer susceptibility

推荐理由: 该研究虽聚焦结直肠癌，但其大规模基因组学分析方法与转录因子调控机制的研究思路，对血液肿瘤的致病机理与基因调控网络研究具有可借鉴的方法学价值。

摘要: Susceptibility transcription factors (TF) whose DNA bindings are altered by genetic variants regulating colorectal cancer (CRC) risk genes remain poorly defined. Using generalized linear mixed models, we analyze 218 TF ChIP-Seq datasets alongside GWAS data from 100,204 CRC cases and 154,587 controls of East Asian and European ancestries. We identify 51 TFs and TF-cofactor interactions, including VDR-cofactors, as key regulators of CRC risk. Integrating these TF insights with transcriptome-wide…

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111 Quantitative Evaluation of the Geriatric Emergency Department Innovation (GEDI). An Interrupted Time Series Study

推荐理由: 该研究展示了针对老年患者的急诊流程优化（GEDI）能有效缩短留观时间并降低住院率，其多学科协作模式对改善老年患者（包括合并血液系统疾病的患者）的急诊处置效率具有直接的实践参考价值。

摘要: CONCLUSION: The introduction of GEDI reduced EDLOS and inpatient admissions in older patients. Further research on economic benefit, patient-related outcome, and experience measures are needed to further evaluate the success of this program.

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112 Acceptability of treadmill perturbation-based balance training in older adults at risk of falling: A mixed-methods evaluation of participant and trainer perspectives

推荐理由: 该研究虽非直接针对血液疾病，但对关注老年血液病患者跌倒风险及康复干预的临床工作者有间接参考价值，有助于理解如何提升高风险人群对训练干预的接受度。

摘要: CONCLUSIONS: Treadmill PBT were generally well accepted by trainers and older adults at risk of falling but showed lower acceptability among participants with fall history. Implementing PBT in individuals with no fall history may help mitigate anxiety related to prior fall experiences and support higher acceptability.

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113 Natural Killer T cells promote cholestatic liver disease independent of antigen presentation by cholangiocytes

推荐理由: 该研究揭示了NKT细胞在胆汁淤积性肝病中不依赖CD1d的新机制，为血液学中免疫细胞介导的疾病机制提供了可借鉴的病理生理视角。

摘要: CONCLUSIONS: A subset of hepatic NKT cells contributes to cholestatic liver disease, and their activation is independent of CD1d antigen presentation on cholangiocytes.

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114 Daily briefing: Why ‘harmless’ germs can be deadly for some people

推荐理由: 由于未提供摘要内容，无法对文章的研究价值、社会影响或血液疾病相关性进行具体评估，建议读者在获取全文后再行判断是否值得深入阅读。

摘要: No abstract

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115 LLMs behaving badly: mistrained AI models quickly go off the rails

推荐理由: 由于未提供摘要，无法评估文章的具体内容与价值。若标题或主题涉及血液疾病，血液科研究人员仍可检索全文以判断是否具有参考意义，但基于当前信息无法给出针对性推荐。

摘要: No abstract

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116 Six steps to protect researchers’ digital security

推荐理由: 因缺少文章摘要，无法评估其具体内容与研究价值，建议提供摘要后再进行针对性评价。

摘要: No abstract

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117 Distributed multitudes of bio-inspired, biodegradable Lagrangian sensors for environmental sustainability

推荐理由: 由于未提供摘要，无法评估文章的具体内容与价值，建议先获取全文或摘要信息以判断其是否值得阅读。

摘要: No abstract

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118 Fabry Disease Cardiomyopathy: A Practical Guide for Cardiologists

推荐理由: 该文章对法布里病这一溶酶体贮积症进行了概述，有助于了解相关病理机制，但其内容未突出血液学表现或直接关联，对血液疾病研究的可借鉴性较为有限。

摘要: Fabry disease (FD) is an X-linked lysosomal storage disease that results in the accumulation of glycosphingolipids, such as globotriaosylceramide (Gb3) in a variety of cells. FD most prominently involves cardiac, nervous, and renal tissue, with cardiac complications representing the most common cause of death. Fabry disease has a prevalence ranging between 1:8454 to 1:117,000 among men. The higher prevalence included patients with the A143T mutation, which was shown to be a non-disease causing…

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119 Humanoid robots step up their game: how useful are the latest droids?

推荐理由: 该摘要缺失具体内容，无法评估其学术或应用价值，仅可给予中性基准分。建议补充完整信息后再进行评判。

摘要: No abstract

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120 Gradient nanofiber aerogels for extreme cryogenic and thermal environments

推荐理由: 该文章在仿生多梯度结构设计和柔性压力传感器制备方法上具有显著创新性与严谨性，为材料科学和传感器领域提供了可借鉴的先进策略，但直接与血液疾病研究的关联性较弱，仅可作为跨学科方法参考。

摘要: Striking a balance between sensitivity and detection range while ensuring stability under extreme temperatures remains a formidable challenge in the design of flexible pressure sensors. To address this issue, we draw inspiration from the multi-gradient architectures of nature and propose a bottom-up self-assembly strategy. By leveraging a meticulously orchestrated multi-step approach that encompasses electrospinning, sequential freezing, and thermal imidization, we successfully fabricate a…

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121 Reduction of RAD23A extends lifespan and mitigates pathology in a mouse model of TDP-43 proteinopathy

推荐理由: 本文虽聚焦神经退行性疾病中蛋白质错误折叠的机制探讨，研究方法与思路（如修饰基因筛选）对血液病领域中异常蛋白聚集相关研究（如淀粉样变性、某些骨髓瘤并发症）有一定可借鉴性，但直接关联度有限，适合拓展交叉学科视野时选择性阅读。

摘要: Protein misfolding and aggregation are cardinal features of neurodegenerative disease (NDD) and they contribute to pathophysiology by both loss-of-function (LOF) and gain-of-function (GOF) mechanisms. This is well exemplified by TDP-43 which aggregates and mislocalizes in several NDDs. The depletion of nuclear TDP-43 leads to reduction in its normal function in RNA metabolism and the cytoplasmic accumulation of TDP-43 leads to aberrant protein homeostasis. A modifier screen found that loss of…

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122 Reentrant melting of scarred odd crystals by self-shear

推荐理由: 该研究在软物质物理和活性物质领域具有方法学创新性，揭示了受限几何中拓扑缺陷对奇数弹性体系集体动力学的影响，但内容与血液疾病研究无直接关联，仅对研究细胞群体行为或生物物理模型有间接参考价值。

摘要: Spatial confinement can induce geometrical frustration in condensed phases, giving rise to topological defects that confer materials with new and exotic properties. Here, we experimentally uncover the effect of confinement-induced defect strings termed ‘grain boundary scars’ on the behavior of dense two-dimensional assemblies of granular spinners, a canonical odd elastic solid. The spatial arrangement of these scars fundamentally reshapes the flows triggered by chiral activity. Specifically,…

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123 Surgical Management of Combined Patellofemoral and Proximal Tibiofibular Joint Instability in a Patient With Ehlers-Danlos Syndrome

推荐理由: 该文章详细描述了埃勒斯-当洛斯综合征患者膝关节双重不稳定的联合手术技术，对外科处理罕见并发症具有参考价值，但内容高度专科化且与血液疾病直接关联较弱。

摘要: Ehlers-Danlos syndrome often presents with patellofemoral instability and, less commonly, proximal tibiofibular joint (PTFJ) instability, leading to compounded symptoms and functional impairment. This Technical Note describes a combined surgical approach for managing dual instability in patients with Ehlers-Danlos syndrome, involving medial patellofemoral ligament reconstruction and tibial tubercle osteotomy, in conjunction with PTFJ stabilization using an adjustable-loop cortical suspension…

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124 Identifying patients at risk in revision arthroplasty: a comprehensive single-centre analysis

推荐理由: 该研究虽未直接涉及血液疾病，但其对围手术期感染、高龄及合并症管理的风险分析，对血液科患者（如凝血障碍、免疫抑制者）的围术期评估具有间接参考价值。

摘要: CONCLUSIONS: Infection (in all revisions), periprosthetic fractures (in rTKA), advanced age, and high comorbidity burden were critical determinants of perioperative adverse events and hospital utilisation. Focused perioperative care strategies targeting these risk groups are essential to mitigate adverse outcomes and optimise healthcare resources.

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125 Tailoring polarization homogeneity in discontinuous-columnar Bi(Fe,Mn)O<sub>3</sub> thin films via dislocation engineering with controlled self-assembly

推荐理由: 该文章在铁电薄膜缺陷调控方法学上具有显著创新性和技术严谨性，但内容与血液疾病研究无直接关联，仅建议关注材料科学或固态物理交叉领域的研究者参考其微观结构控制思路。

摘要: Defect microstructures, particularly dislocations, are pivotal in determining the functional properties of ferroelectric thin films. While their density is routinely considered, the critical role of their spatial configuration has remained largely unexplored and difficult to control. Here, we develop a controlled self-assembly strategy for dislocations in Bi(Fe,Mn)O(3) thin films on Ni-Cr that directly addresses this challenge. By employing a LaNiO(3) buffer layer, we template a…

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126 Light-driven restructuring generates nanoisland NiIr alloy for efficient methane dry reforming

推荐理由: 该研究在光催化甲烷干重整领域展示了创新的纳米合金催化剂设计与原位表征方法，具有较高的材料科学参考价值，但与血液疾病研究无直接关联，仅可作为交叉学科方法学的间接借鉴。

摘要: The simultaneous utilization of methane and carbon dioxide via dry reforming holds promise for sustainable syngas production, yet conventional thermocatalytic processes suffer from energy-intensive operation and catalyst deactivation. Here, we report a light-driven methane dry reforming strategy utilizing sinter-resistant nano-island alloys catalyst, which are dynamically evolved from partially oxidized NiIr nanoclusters anchored on TiO(2) under photoexcitation. In situ characterization reveals…

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127 Advances, challenges and prospects of origami and kirigami optoelectronics

推荐理由: 该文章主要聚焦于可变形光电器件的基础材料与结构设计创新，对血液疾病研究的直接关联性较弱，更适合材料科学或柔性电子领域的研究者参考；若未来应用于生物传感或医疗设备开发，或可间接助力血液检测技术发展。

摘要: Switchable between 2D and 3D forms, origami and kirigami have emerged as a versatile platform for deformable optoelectronic devices. Unlike electronic devices, whose electrical properties remain largely unchanged during transformation, the optoelectronic properties of these systems can vary significantly, offering opportunities for optical tunability but also introducing design challenges. Developing such devices requires careful consideration of electrical, mechanical, and optical factors. This…

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128 Brain-gonad axes harmonize male mating drive and reproductive strategy via steroid hormones

推荐理由: 该研究在神经-生殖轴机制上有创新发现且方法严谨，但对血液疾病研究直接关联较弱，仅在高水平激素对血细胞或骨髓的潜在调控方面存在间接参考价值，血液病研究者可选择性阅读。

摘要: The interplay between the nervous and reproductive systems, encapsulated by the brain-gonad axis, is pivotal for reproduction. However, it remains largely unclear how hormones released from reproductive system actively regulate male sexual behaviors and mating strategies. Here we find that sexual priming induces an elevated sexual drive in both male mice and Drosophila, triggered by the surge of circulating steroid hormones 20-hydroxyecdysone (20E) in flies and testosterone in mice,…

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129 Construction of remote dual stereocenters by electrochemical cobalt-catalyzed enantioselective desymmetrization

推荐理由: 该论文专注于不对称合成化学的机理与方法学创新，虽在有机合成领域具有较高学术价值，但与血液疾病研究无直接关联，对血液病学者借鉴意义有限。若研究方向涉及手性药物合成或立体化学控制，可选择性参考。

摘要: The enantio- and diastereoselective construction of two stereogenic centers represents a highly attractive objective in synthetic chemistry. Extensive asymmetric catalytic methods have been developed for the formation of vicinal stereocenters. In contrast, the simultaneous construction of two constitutionally distinct stereogenic centers at remote positions in a single asymmetric catalytic step remains very scarce, owing to the lack of reliable models for distant stereochemical induction for…

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130 Near-infrared-driven photocatalytic CO<sub>2</sub> reduction to C<sub>2</sub> hydrocarbons by bis(terpyridine)-metal functionalized lead halide frameworks

推荐理由: 该文章在光催化材料设计方面具有较高创新性和方法学严谨性，但研究对象为无机-有机杂化铅卤化物，与血液疾病研究无

摘要: Hybrid lead halides have demonstrated great promise for CO(2) photoreduction due to their excellent photophysical properties. However, developing a single-component hybrid lead halide that combines high intrinsic stability with near-infrared absorption remains a significant challenge. Herein, to address both issues, we design a series of nine highly stable lead halide frameworks functionalized by bis(terpyridine)-metal complexes, which exhibit strong light absorption extending into the…

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131 Cluster level entropy enhancement in neutral acetate electrolytes enables economical and durable zinc air batteries

推荐理由: 该文虽聚焦锌空气电池电解质研究，与血液疾病无直接关联，但其对锌离子浓度平衡机制的探讨或可为某些锌代谢相关血液病（如锌缺乏性贫血）的病理

摘要: Neutral zinc-air batteries provide a viable alternative to alkaline systems by avoiding salt creep and carbonate passivation. Among candidate electrolytes, acetate-based formulations are particularly attractive for their low cost, sustainability, and compatibility with ambient-air operation. However, their widespread adoption is limited by a trade-off between two concentration regimes. Dilute electrolytes trigger side reactions and lack ionic strength, while concentrated ones suffer from kinetic…

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132 Unveiling the metallogenic continuum of an Archean craton

推荐理由: 本文在地质成矿系统研究方面具有较高创新性和方法论严谨性，但内容与血液疾病无直接关联，仅对研究地球历史时期元素富集机制有专业参考价值，不建议血液学研究者优先阅读。

摘要: The synchronous formation between 2675-2655 Ma of hydrothermal gold mineralisation in the Kalgoorlie and Kurnalpi Terranes and of magmatic sulfide mineralisation enriched in nickel, copper and platinum-group elements in the South West Terrane of the Archean Yilgarn Craton of Western Australia offers key insights into first-order controls on the genesis of mineral systems. Here we show that hydrothermal and magmatic deposits formed on opposite sides of this craton share four key features: timing,…

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133 Characteristics and prognostic significance of myelodysplasia-related features in VEXAS syndrome

推荐理由: 由于未提供摘要内容，无法评估研究的创新性、社会影响力或具体血液病相关性，因此不推荐阅读。建议提供摘要信息以便进行有效评估。

摘要: No abstract

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134 Comparative visual and refractive analysis of two enhanced monofocal intraocular lenses

推荐理由: 由于未提供摘要，无法评估文章的具体内容与价值，建议在获取全文或摘要后再判断是否适合阅读。

摘要: No abstract

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