追觅 - 2026-01-23

生成时间: 2026−01-23 22:38

分析文章数量: 80

1 Immunomodulatory effect of dasatinib plus blinatumomab versus ponatinib plus blinatumomab in newly diagnosed Ph+ acute lymphoblastic leukemia

推荐理由: 该研究针对Ph+急性淋巴细胞白血病的前沿联合疗法进行了深入的免疫调节机制探索，通过大样本、多时间点的免疫细胞动态分析，为优化靶向-免疫联合治疗策略提供了关键数据参考，对血液肿瘤临床与转化研究具有重要借鉴价值。

摘要: In Ph+ acute lymphoblastic leukemia, frontline dasatinib plus blinatumomab (dasa+blina) is associated with long-term survival rates of 75-80%. The phase III GIMEMA ALL2820 trial has explored ponatinib with blinatumomab (pona+blina). In the present study, the immune modulation induced by dasa+blina and pona+blina was investigated. Immune cells were analyzed at the end of induction (T0) and after 2, 4 and 5 blinatumomab cycles (T2, T4, T5). Among 153 patients (43 dasa+blina, 110 pona+blina), the…

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2 A transgenic mouse model for reticulated platelet detection reveals expansion after myocardial ischemia/reperfusion

推荐理由: 该研究创新性地利用基因工程小鼠模型揭示网织血小板在心肌缺血再灌注损伤中的主动作用机制，突破了其仅作为生物标志物的传统认知，对血小板相关血液疾病研究和治疗策略具有重要借鉴价值。

摘要: Reticulated platelets are newly formed, RNA-rich platelets with heightened reactivity. Although elevated levels are observed after myocardial ischemia/reperfusion injury, their functional contributions to postischemic pathology remains poorly defined. We aimed to determine whether reticulated platelets actively contribute to inflammation and repair following myocardial ischemia and reperfusion, rather than serving solely as biomarkers of platelet turnover. We generated Pf4-Cre:RiboTag mice, in…

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3 Beyond metabolism: exploring the regulatory and therapeutic implications of lactate and lactylation in cancer-regulated cell death

推荐理由: 该文章系统阐述了乳酸及乳酸化修饰在调控肿瘤细胞死亡中的关键作用，为血液系统恶性肿瘤（如白血病、淋巴瘤）的耐药机制和靶向治疗策略提供了新的分子视角与潜在干预靶点，具有较高的借鉴价值。

摘要: Lactate, a key byproduct of glycolysis in tumor cells, has emerged as more than just a metabolic waste product. Increasing evidence reveals that lactate and its associated post-translational modification (PTM), lactylation, play multifaceted roles in regulating various forms of regulated cell death (RCD), thereby contributing to cancer proliferation, therapy resistance, and immune exclusion. Notably, evasion of RCD is a hallmark of cancer and targeting RCD may represent a promising therapeutic…

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4 Microbial metabolite FAD mobilizes adipocyte lipid remodeling to enhance cancer immunotherapy efficacy

推荐理由: 该研究揭示了肥胖患者肠道微生物通过核黄素代谢影响免疫检查点抑制剂疗效的新机制，其发现的微生物-代谢物-免疫轴为肿瘤免疫治疗及代谢相关血液疾病研究提供了新颖且可借鉴的思路。

摘要: Crosstalk between gut microbiota and adipose tissue critically shapes immunotherapy responses in patients with cancer. An obesity-associated microbial signature enriched in riboflavin-producing taxa was identified, along with increased microbial riboflavin biosynthesis pathway and elevated levels of flavin adenine dinucleotide (FAD), in obese responders to immune checkpoint blockade (ICB). In diet-induced obese (DIO) mice, fecal microbiota transplantation (FMT), administration of Lachnospiraceae…

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5 “Leukemic pernio”: perniosis-like presentations of chronic lymphocytic leukemia

推荐理由: 该文章报告了慢性淋巴细胞白血病（CLL）一种罕见且易被误诊的皮肤表现——冻疮样皮损，具有重要的临床警示价值，提醒血液科及皮肤科医生在遇到不典型皮损时需考虑潜在血液系统恶性肿瘤的可能。

摘要: Chronic lymphocytic leukemia (CLL) is the most common type of leukemia in adults. Skin involvement by malignant CLL cells, or leukemia cutis (LC), typically presents as localized or generalized papulonodular lesions. Here we report a series of five patients with CLL who presented with perniosis-like skin findings on the ears, nose, and/or digits. Histopathologic analysis of seven skin specimens from these five patients showed a relatively dense, nodular to diffuse, pan-dermal infiltrate of…

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6 Metachronous Presentation of BCL-2-Positive Lung Adenocarcinoma and Acute Myeloid Leukemia: A Case Presentation and Review of the Literature

推荐理由: 该病例报告创新性地展示了功能精准医学工具（MiniPDX）在难治性AML治疗中的直接应用价值，并为跨肿瘤BCL-2靶向治疗策略提供了独特的临床思路，对血液科医生处理复杂耐药病例具有直接参考意义。

摘要: CONCLUSION: This salvage regimen successfully induced a second CR in the refractory AML. This case highlights the complexity of managing these distinct malignancies, where the lung tumor’s BCL-2 expression provided a rationale for the AML therapy. Furthermore, the MiniPDX assay proved valuable in guiding personalized therapy for refractory disease, demonstrating the potential of functional precision medicine strategies.

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7 Hypoxic glycolysis-driven histone lactylation activates NHE7 to promote endometrial cancer progression via COX6C-mediated endoplasmic reticulum stress

推荐理由: 该研究揭示了组蛋白乳酸化修饰在缺氧微环境中促进肿瘤恶性进展的新机制，其发现的代谢-表观遗传调控轴对研究白血病等血液肿瘤的代谢适应与耐药性具有重要的借鉴价值。

摘要: CONCLUSION: Hypoxia-driven glycolysis induces histone lactylation, leading to the upregulation of NHE7 expression. This process enhances OXPHOS-induced ER stress by upregulating COX6C expression, ultimately contributing to the malignant progression of EC.

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8 Beyond metabolism: exploring the regulatory and therapeutic implications of lactate and lactylation in cancer-regulated cell death

推荐理由: 该文章系统阐述了乳酸及乳酸化修饰在调控肿瘤细胞死亡中的关键作用，为血液肿瘤（如白血病、淋巴瘤）的代谢靶向治疗提供了新的机制视角和潜在干预策略，对血液病研究具有重要借鉴价值。

摘要: Lactate, a key byproduct of glycolysis in tumor cells, has emerged as more than just a metabolic waste product. Increasing evidence reveals that lactate and its associated post-translational modification (PTM), lactylation, play multifaceted roles in regulating various forms of regulated cell death (RCD), thereby contributing to cancer proliferation, therapy resistance, and immune exclusion. Notably, evasion of RCD is a hallmark of cancer and targeting RCD may represent a promising therapeutic…

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9 Integrative analysis identifies lactylation-related biomarkers in sepsis via bioinformatics and machine learning

推荐理由: 该研究揭示了乳酸代谢与免疫功能障碍在脓毒症中的新机制，鉴定的乳酸化相关生物标志物对脓毒症的早期诊断和血液免疫研究具有直接参考价值。

摘要: CONCLUSION: This multi-disciplinary study identifies SLC4A4, SLC16A4, and CRYAB as promising lactylation-related diagnostic biomarkers for sepsis. Our findings indicate the intricate interplay between lactate metabolism, epigenetic modification, and immune dysfunction in sepsis, providing new insights for early diagnosis and mechanistic investigation.

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10 AOC3 Loss Promotes Imatinib Resistance in GIST by Stabilizing HK2 and Enhancing H3K18la-Driven Myc Transcription

推荐理由: 该研究揭示的AOC3下调通过稳定HK2增强糖酵解导致耐药的机制，为理解酪氨酸激酶抑制剂耐药提供了全新视角，其涉及的代谢重编程通路在血液系统肿瘤（如CML）中具有高度可借鉴性。

摘要: Gastrointestinal stromal tumor (GIST) frequently develops resistance to imatinib (IM). This study identifies AOC3 downregulation as a critical contributor to IM resistance. Analyzing clinical samples and IM-sensitive/resistant GIST cell lines, we found AOC3 significantly decreased in resistant states. Functionally, AOC3 knockdown promoted IM resistance, enhanced glycolytic activity, and increased lactate production. Mechanistically, AOC3 loss attenuated ubiquitin-mediated degradation of HK2,…

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11 Assimilative causal inference

推荐理由: 该文提出的同化因果推理（ACI）方法在解决高维动态系统的瞬时因果关系识别上具有方法论创新，对于研究血液疾病中复杂的多因素动态相互作用（如凝血机制、肿瘤微环境演变）提供了新的分析工具借鉴思路。

摘要: Causal inference is fundamental across scientific disciplines, yet existing methods struggle to capture instantaneous, time-evolving causal relationships in complex, high-dimensional systems. In this paper, assimilative causal inference (ACI) is developed, which is a methodological framework that leverages Bayesian data assimilation to trace causes backward from observed effects. ACI solves the inverse problem rather than quantifying forward influence. It uniquely identifies dynamic causal…

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12 Longitudinal plasma proteomic signatures of elite and viremic spontaneous HIV controllers

推荐理由: 该研究通过对HIV精英控制者这一独特人群进行深度血浆蛋白质组学分析，不仅为病毒免疫控制机制提供了新见解，其高通量生物标志物筛选方法和免疫调节相关通路的发现对血液肿瘤及免疫缺陷

摘要: A small percentage of people living with HIV (PLHIV) spontaneously regulate viral replication without suppressive antiretroviral treatment (ART) and are categorized into ‘elite controllers’ (EC, HIV-RNA < 50 c/mL) and ‘viremic controllers’ (VC, HIV-RNA between 50-10,000 c/ml). Some EC and VC may lose controller status in time. Here we provide extensive plasma proteomics to identify biomarkers and pathways related to spontaneous viral control and its long-term preservation among 36 EC and 147 VC…

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13 Integrative analysis identifies lactylation-related biomarkers in sepsis via bioinformatics and machine learning

推荐理由: 该研究创新性地揭示了脓毒症中乳酸代谢与免疫紊乱的表观遗传调控机制，提出的生物标志物对早期诊断和血液免疫相关研究具有直接借鉴价值。

摘要: CONCLUSION: This multi-disciplinary study identifies SLC4A4, SLC16A4, and CRYAB as promising lactylation-related diagnostic biomarkers for sepsis. Our findings indicate the intricate interplay between lactate metabolism, epigenetic modification, and immune dysfunction in sepsis, providing new insights for early diagnosis and mechanistic investigation.

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14 Atypical pericapillary Ly6G⁺Nur77⁺ macrophages initiate type-2 immune responses to allergens in the mouse lung

推荐理由: 该研究发现的Ly6G⁺Nur77⁺巨噬细胞亚群模糊了传统髓系细胞分类界限，其独特的表型与抗原提呈功能为理解血液系统疾病中髓系细胞的异质性、可塑性及免疫应答起始机制提供了新的

摘要: The mechanisms of airway allergen sensing and type 2 immune response initiation remain poorly understood. Using a mouse house dust mite (HDM)-induced allergic airway model, we identify a population of lung macrophages located close to alveolar capillaries that express Ly6G and the nuclear receptor Nr4a1/Nur77. These atypical Ly6G^(+)Nur77^(+) macrophages preferentially capture airway-delivered allergens and play an important role in initiating HDM-driven T helper type 2 (Th2) responses. They…

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15 Identification of monogenic variants in steroid-resistant and steroid-sensitive nephrotic syndrome

推荐理由: 该研究明确了遗传因素在SRNS（激素抵抗型肾病综合征）中的诊断价值，对于研究肾病相关贫血、凝血障碍等血液并发症具有间接参考意义，尤其有助于理解继发性血液异常的遗传背景。

摘要: CONCLUSIONS: A genetic cause was established in 19.1% of SRNS patients (15.8% in known SRNS genes and 3.3% in phenocopy genes), but only 1.9% of non-steroid-resistant cases.

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16 Click. Screen. Degrade. A Miniaturized D2B Workflow for Rapid PROTAC Discovery

推荐理由: 该文章介绍了一种突破PROTACs传统合成瓶颈的高通量工作流程，其技术思路对于推动血液肿瘤靶向降解药物的研发具有重要的方法论借鉴价值。

摘要: Targeted protein degradation is one of the fastest developing fields in medicinal chemistry and chemical biology. Despite significant development in assay technologies and inhibitor discovery, the development of PROTACs remains a challenging endeavor since rational design approaches remain widely elusive. Our workflow eliminates the rate-limiting step of classic synthesis, namely compound purification, and pairs it with high-throughput, semi-automated plate-based synthesis, and direct cellular…

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17 Structural decoding of reversible covalent linkage of odorants in human olfactory receptor OR6A2

推荐理由: 该研究通过反向突变策略在嗅觉受体结构解析上取得重要突破，并揭示了OR6A2在巨噬细胞炎症反应中的调控作用，为血液系统炎症相关疾病的研究提供了新的分子视角和潜在靶点。

摘要: Olfactory receptors (ORs) are a diverse superfamily of G protein-coupled receptors responsible for odor detection that are also implicated in non-olfactory physiological functions. OR6A2, a class II OR, selectively senses medium-chain aldehydes and belongs to a cluster of ORs genetically associated with the “soapy” perception of cilantro. It also modulates macrophage-mediated inflammatory responses. Structural studies of ORs have long been challenging. Using a back-mutation strategy, we…

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18 Hypoxic glycolysis-driven histone lactylation activates NHE7 to promote endometrial cancer progression via COX6C-mediated endoplasmic reticulum stress

推荐理由: 该研究揭示了缺氧环境下组蛋白乳酸化修饰通过代谢重编程促进肿瘤恶性进展的新机制，为血液系统肿瘤（如白血病、淋巴瘤）中代谢与表观遗传交叉调控的研究提供了可借鉴的思路。

摘要: CONCLUSION: Hypoxia-driven glycolysis induces histone lactylation, leading to the upregulation of NHE7 expression. This process enhances OXPHOS-induced ER stress by upregulating COX6C expression, ultimately contributing to the malignant progression of EC.

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19 Distal enhancer RNAs regulate alternative promoter usage of MYB and drug resistance in human leukemia cells

推荐理由: 该研究首次揭示了MYB基因上游增强子区双向eRNAs在白血病发生及耐药中的调控机制，为靶向MYB转录网络克服白血病治疗抵抗提供了新的潜在靶点，对血液肿瘤基础与转化研究具有重要借鉴价值。

摘要: MYB is a master transcription factor for the hematopoietic system, and its dysregulation drives the development and therapy resistance of leukemia. However, the mechanisms of MYB regulation and MYB-related therapy resistance are still unclear. Here, we identified two bidirectional enhancer RNAs (eRNAs), MY34UE-AS and MY34UE-S, transcribed from the −34 kb enhancer region of MYB. Both eRNAs promote MYB transcription, proliferation, and migration in human leukemia cells, although through different…

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20 A common DNA deletion altering the 3′UTR of mdr1 is associated with reduced mefloquine susceptibility in P. vivax parasites from Cambodian patients

推荐理由: 该研究通过基因组测序揭示了间日疟原虫耐药性相关的基因缺失特征，对理解疟疾耐药机制及指导临床抗疟治疗具有直接参考价值，血液病领域研究者可借鉴其方法学与发现。

摘要: Artemisinin-combination therapies (ACTs) are now recommended for the treatment of uncomplicated malaria caused by Plasmodium vivax, the parasite responsible for the majority of malaria infections outside of Africa. We sequence the genomes of 206 P. vivax parasites collected from Cambodian malaria patients and show that more than 80% of them carry a DNA deletion located immediately downstream of the multidrug resistance 1 gene (mdr1) protein-coding sequence. This 837 bp deletion overlaps with a…

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21 Homotypic membrane-powered electrochemical microfluidic analysis of extracellular vesicles for precise cancer diagnosis

推荐理由: 该文章提出了一种基于同源癌细胞膜的仿生界面新技术，用于高特异性捕获与分析细胞外囊泡，其创新性的生物膜工程策略具有显著的方法学参考价值。虽然研究以乳腺癌为模型，但其对EVs的精准分析思路可直接借鉴于血液肿瘤（如淋巴瘤、白血病）来源的囊泡研究，为液体活检和肿瘤分型提供了新的技术路径。

摘要: The cancer cell membrane, rich in tumor-associated antigens, effectively replicates the characteristics of cancer cells by presenting specific protein profiles. Leveraging the inherent binding capability of the cancer cell membrane, here we show a homotypic membrane-powered biomimetic interface for subtype-specific analysis of breast cancer extracellular vesicles (EVs). Specifically, the breast cancer cell membrane is employed to coat a gold substrate, thereby forming the biomimetic interface…

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22 Evaluating single-cell ATAC-seq atlasing technologies using sequence-to-function modeling

推荐理由: 该研究通过系统评估scATAC-seq数据集构建标准与平台适用性，为解码增强子调控逻辑提供了重要的方法学参考，其建模思路可借鉴于血液细胞分化和疾病相关表观遗传机制研究。

摘要: Deciphering the cis-regulatory logic underlying cell type identity remains a key challenge in biology. Single-cell chromatin accessibility (scATAC-seq) atlases enable training of sequence-to-function (S2F) deep learning models to decode enhancer logic. Yet, optimal criteria for constructing training datasets, i.e., the number of cells and ATAC fragments, remain unclear. Moreover, the suitability of different scATAC-seq platforms for such models has not been systematically tested. We introduce…

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23 Phosphorothioate DNA modification by BREX type 4 systems in the human gut microbiome

推荐理由: 该研究首次系统揭示了人类肠道微生物组中磷酸硫酰化修饰基因的分布与新型合成系统，为理解微生物表观遗传调控及宿主-菌群互作提供了新视角，对研究菌群相关炎症或免疫性血液疾病具有潜在借鉴价值。

摘要: Among dozens of microbial DNA modifications regulating gene expression and host defense, phosphorothioation (PT) is the only known backbone modification, with sulfur inserted at a non-bridging oxygen by dnd and ssp gene families. Here we explored the distribution of PT genes in 13,663 human gut microbiome genomes, finding that 6.3% possessed dnd or ssp genes predominantly in Bacillota, Bacteroidota, and Pseudomonadota. This analysis revealed several previously undescribed PT synthesis systems,…

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24 Antibody-conjugated mesenchymal stromal cell drug delivery system for the treatment of autoimmune diseases in mice

推荐理由: 该研究创新性地将抗体-药物偶联物（ADC）策略与间充质基质细胞（MSCs）疗法结合，提出了针对自身免疫性疾病的新型靶向递送系统，其设计思路对血液领域免疫相关疾病（如自身免疫性溶血性贫血等）的治疗策略具有重要借鉴价值。

摘要: Autoimmune diseases (AIDs), such as psoriasis and rheumatoid arthritis, are driven by immune dysfunction, leading to chronic inflammation and tissue damage. Mesenchymal stromal cells (MSCs) possess immunomodulatory and tissue repair properties; however, the therapeutic applicability of MSCs faces limitations due to the low homing efficiency upon systemic infusion. Inspired by antibody-drug conjugates (ADC), here we develop an antibody-conjugated MSC-based drug delivery system (AcM-DDS) that…

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25 Meta-unstable mRNAs in activated CD8<sup>+</sup> T cells are defined by interlinked AU-rich elements and m<sup>6</sup>A mRNA methylation

推荐理由:

摘要: CD8^(+) T cells can rapidly produce effector molecules following activation. This activation triggers rapid changes in gene expression that rely on the control of mRNA levels via multiple mechanisms, including RNA modifications. N⁶-methyladenosine (m⁶A) is an abundant post-transcriptional modification that promotes the decay of messenger RNAs in the cytosol. However, how recognition of m⁶A sites is integrated with other regulatory mechanisms that alter the fate of immunoregulatory mRNAs in…

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26 AOC3 Loss Promotes Imatinib Resistance in GIST by Stabilizing HK2 and Enhancing H3K18la-Driven Myc Transcription

推荐理由: 该研究虽聚焦胃肠道间质瘤，但其揭示的AOC3下调通过糖代谢重编程（HK2稳定化）导致伊马替尼耐药的机制，

摘要: Gastrointestinal stromal tumor (GIST) frequently develops resistance to imatinib (IM). This study identifies AOC3 downregulation as a critical contributor to IM resistance. Analyzing clinical samples and IM-sensitive/resistant GIST cell lines, we found AOC3 significantly decreased in resistant states. Functionally, AOC3 knockdown promoted IM resistance, enhanced glycolytic activity, and increased lactate production. Mechanistically, AOC3 loss attenuated ubiquitin-mediated degradation of HK2,…

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27 High-definition likelihood inference of genetic colocalization reveals protein biomarkers for human complex diseases

推荐理由: 该研究提出的遗传共定位信号检测框架方法严谨、通用性强，可为血液病致病机制研究与靶点筛选提供重要的方法论参考。

摘要: CONCLUSION: This research establishes a robust framework for detecting genetic colocalization signals, enabling the prioritization of disease-relevant protein targets and informing therapeutic development strategies.

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28 Interfacial Behavior, Supramolecular Assembly, and Photophysics of Phospholipid-Porphyrin and Phospholipid-Phthalocyanine Conjugates

推荐理由: 该研究报道的新型磷脂-卟啉/酞菁锌复合物，在超分子组装和界面行为方面具有创新性，其卟啉类结构与血红素相似，为血液疾病相关的光动力治疗或血红蛋白仿生研究提供了可借鉴的设计思路。

摘要: The design of supramolecular assemblies based on phospholipid-porphyrinoid conjugates has attracted growing attention in recent years, particularly for biomedical applications. Here, we report two novel amphiphilic conjugates, ZnPor(PL)(4) and ZnPc(PL)(4,) obtained by coupling four molecules of 1-myristoyl-2-(14-carboxymyristoyl)-sn-glycero-3-phosphocholine to zinc(II)-tetraaminophenylporphyrin or zinc(II)-tetraaminophthalocyanine, respectively. Their interfacial behavior, their supramolecular…

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29 Molecular characterization of cell decay in inflammation and topological assignment of released cfDNA for integrative laboratory and radiological outcome assessment

推荐理由: 该研究通过自动化凝胶电泳分析cfDNA完整性指数，创新性地整合实验室与影像学资源，对预测ARDS等临床结局具有潜在应用价值，其方法学思路对血液病领域cfDNA相关研究具有可借鉴的跨学科参考意义。

摘要: CONCLUSION: Implementing the cfDNA integrity index on an automated gel electrophoresis demonstrated promising results in predicting clinical outcomes like ARDS occurrence and mortality. Therefore, integrating laboratory and imaging resources could enhance the allocation of optimal care and may identify secondary systemic complications, especially liver involvement, as our results suggest reduced lead-time for detecting liver injury.

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30 SpatialProp: tissue perturbation modeling with spatially resolved single-cell transcriptomics

推荐理由: 该文章聚焦于前沿的遗传扰动单细胞技术，对血液疾病机制研究具有方法学上的重要借鉴意义，尤其适用于探索造血发育或恶性血液病的因果调控网络。

摘要: Perturbational studies are the gold standard for identifying causal relationships between components of biological systems. Recent technological advances, including Perturb-seq and related assays, have enabled high-throughput screening of genetic perturbation effects on single cells. Several machine learning tools have also been developed to infer the effect of single-cell perturbations. However, both approaches are generally limited to dissociated cells, and the effect of genetic perturbations…

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31 Identification of Or5v1/Olfr110 as an oxylipin receptor and anti-obesity target

推荐理由: 该研究创新性地开发了ARIG方法并发现了嗅觉受体Or5v1/Olfr110可作为oxylipin受体，为代谢性疾病与受体信号转导研究提供了新工具

摘要: Oxylipins are important metabolic messengers for normal life activities, and olfactory receptors (ORs) are known for their low affinity for odor and are not considered oxylipin receptors. By developing the “anonymous receptor identification by reverse-G-protein pull-down” (ARIG) method, we identify orphan OR Or5v1/Olfr110 as an oxylipin 12(S)-hydroxyeicosapentaenoic acid (12(S)-HEPE) receptor. The serum from obese patients with increased BMI showed lower Or5v1/Olfr110-Gs activation compared with…

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32 A novel dual histone mark reader ZCWPW2 regulates meiotic recombination through lactylation and transcriptional regulation in humans and mice

推荐理由: 该研究揭示了减数分裂中组蛋白修饰读写系统的新调控机制，对理解基因组稳定性及血液肿瘤中的染色体异常具有潜在借鉴价值。

摘要: Meiotic recombination ensures accurate chromosome segregation and genetic diversity during gametogenesis, and its disruption leads to infertility. The dual histone methylation writer-reader system, in which PRDM9 deposits H3K4me3 and H3K36me3 marks at nucleosomes to define recombination hotspots and ZCWPW1 acts as a reader recognizing these marks, is essential for meiotic recombination. However, the regulatory mechanisms of this system remain unclear. Here, we showed that deficiency of ZCWPW2…

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33 Outsourcing of energetically costly amino acids at the origin of animals

推荐理由: 该研究从进化角度揭示了必需氨基酸代谢简化的起源，虽非直接针对血液疾病，但其对代谢通路的深入解析可为贫血、代谢性血液病等研究提供重要的进化生物学借鉴。

摘要: Animals are generally capable of synthesizing eleven amino acids, while the remaining nine-often referred to as essential-must be acquired through diet. This metabolic trait profoundly shapes animal ecology and evolutionary trajectories, and recent phylogenomic studies indicate that it arose from gene losses at the root of the animal tree. However, it remains unclear which selective forces, if any, directed this far-reaching metabolic simplification. Here, we show that essential amino acids are…

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34 A bacterial defense system targeting modified cytosine of phage genomic DNA

推荐理由: 该研究深入揭示了噬菌体与细菌防御系统间的新型分子互作机制，为理解宿主-病原体共进化及开发新型抗病毒策略提供了关键见解，对感染免疫及微生物学领域具有重要参考价值。

摘要: The evolutionary arms race between bacteria and phages drives the development of bacterial antiviral defense systems and phage counter-defense strategies. Restriction-modification (RM) systems protect bacteria by methylating ‘self’ DNA and cleaving unmodified phage DNA. Phages like T-even coliphages evade RM systems by substituting cytosine with 5-hydroxymethyl cytosine (5hmC) or 5-glucosylated hmC (5ghmC). Here, we characterize ‌a single-component antiviral defense system featuring a GIY-YIG…

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35 Synaptic and intrinsic membrane defects disrupt early neural network dynamics in Down syndrome

推荐理由: 该研究利用等基因iPSC模型深入揭示21三体综合征影响皮层神经网络的电生理机制，其严谨的干细胞分化与电生理记录方法对血液病相关的细胞模型研究具有方法学借鉴价值。

摘要: Down syndrome, caused by trisomy 21, affects around six million people worldwide and features learning, memory and language deficits. However, the mechanisms underlying trisomy 21 neurophenotypes involving human cortical circuitry are unknown. By characterising developing neural network dynamics and single cell excitability profiles, from synaptic and voltage-dependent ion channel behaviour using an isogenic induced pluripotent stem cell-derived neuronal model, we show that trisomy 21 impairs…

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36 Single-cell atlas of human lung aging identifies cell type dyssynchrony and increased transcriptional entropy

推荐理由: 该研究采用先进的单细胞测序技术系统性解析肺衰老的细胞分子变化，其中肺泡内皮细胞的衰老转录特征对研究血液系统相关内皮功能障碍与血管老化具有重要参考价值。

摘要: Age is a major risk factor for lung disease. We characterized the changing cellular, transcriptional, and genomic landscape of human lung aging using single-cell RNA sequencing. We find that lung aging is cell-type dyssynchronous, with alveolar epithelial and endothelial cells exhibiting the greatest transcriptional changes. Among alveolar epithelial cells, aging is associated with a decreased relative proportion of surfactant-expressing SPC^(high) AT2 cells. Among alveolar capillary cells, we…

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37 Ultrathin liquid cells for microsecond time-resolved cryo-EM

推荐理由: 该技术突破了传统冷冻电镜的时间分辨率极限，为捕捉蛋白质动态过程提供了革命性工具，虽未直接涉及血液疾病，但其方法论对血液学中蛋白质结构与功能研究具有重要借鉴意义。

摘要: Microsecond time-resolved cryo-electron microscopy promises to significantly advance our understanding of protein function by rendering cryo-electron microscopy (cryo-EM) fast enough to observe proteins at work. This emerging technique involves flash melting a cryo sample with a laser beam to provide a brief time window during which dynamics are initiated. When the laser is switched off, the sample revitrifies, arresting the proteins in their transient configurations. However, observations have…

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38 Comparative multi-omic analysis reveals conserved and derived mechanisms of fin and limb regeneration

推荐理由: 该研究采用跨物种多组学方法揭示了再生过程的保守机制，其发现的DNA损伤修复与缺氧响应通路对造血干细胞与骨髓再生研究具有潜在借鉴价值。

摘要: Comparative studies of vertebrate appendages offer a powerful framework for uncovering shared components of an ancestral regeneration toolkit. Here, we employed a multi-omics comparative approach leveraging the regenerative capacity of the axolotl, zebrafish, and Polypterus senegalus, a fish capable of full fin regeneration. We identified conserved markers of proximal and distal blastema territories, shared activation of DNA damage repair, hif1a-mediated hypoxia response, and sequential…

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39 ProteoAutoNet: high-throughput co-eluted protein analysis with robotics and machine learning

推荐理由: 该研究通过开发高通量自动化平台显著提升了蛋白质互作分析的效率和规模，为血液疾病相关的蛋白质复合物与分子机制研究提供了重要的方法学参考。

摘要: Co-fractionation mass spectrometry (CF-MS) enables large-scale profiling of endogenous protein-protein interactions, yet CF-MS data generation is of low throughput and therefore predictive models are often limited by the scarcity and limited diversity of high-quality training data. To address this, we present ProteoAutoNet, a robotic experimental platform integrated with a computational workflow for high-throughput CF-MS analysis. This workflow increases the throughput of sample processing from…

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40 Camouflaged nanorobots target and regulate macrophage subcellular organelle crosstalk patterns to promote neural regeneration

推荐理由: 该研究深入探讨巨噬细胞表型转化的亚细胞器互作机制，其揭示的内质网-线粒体代谢对话对理解免疫细胞功能调控具有重要借鉴意义，可间接推动血液系统疾病中巨噬细胞相关病理的研究。

摘要: Macrophage (Mφ) phenotypic transformation is crucial in determining spinal cord injury (SCI) outcomes. However, the suborganelle crosstalk mechanisms-particularly between the endoplasmic reticulum (ER) and mitochondria-that mediate Mφ subgroup conversion during SCI remain underexplored. We aim to integrate niche intervention strategies and omics sequencing to investigate the effects of the metabolic crosstalk between ER stress (ERS) and mitochondria. Subsequently, we develop a dual-targeted…

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41 Cross-ancestry comparison of aptamer and antibody protein measures

推荐理由: 该研究通过大规模多平台蛋白质组学数据对比，揭示了不同检测平台间蛋白质测量的一致性问题，为血液病相关生物标志物研究的实验设计与数据解读提供了重要方法学参考。

摘要: Measures from affinity-proteomics platforms often correlate poorly, challenging interpretation of protein associations with genetic variants and phenotypes. Here, we examine 2157 proteins measured on both SomaScan 7k and Olink Explore 3072 across 1930 participants with genetic similarity to European, African, East Asian, and Admixed American ancestry references. Inter-platform correlation coefficients for these 2157 proteins follow a bimodal distribution (median r = 0.30). We evaluate protein…

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42 CDK2 inhibitor BLU-222 synergizes with CDK4/6 inhibitors in drug resistant breast cancers through p21/p27 induction

推荐理由: 该研究针对CDK抑制剂在乳腺癌耐药模型的联合应用机制，虽然疾病本身非血液病，但其对细胞周期激酶抑制剂的协同作用机制可为血液肿瘤（如淋巴瘤、白血病）的靶向治疗策略提供可借鉴的思路。

摘要: Cyclin-dependent kinase 4/6 inhibitors (CDK4/6i) combined with endocrine therapy are the standard first-line treatment for hormone receptor-positive, HER2-negative (HR+/HER2-) metastatic breast cancer, but resistance inevitably develops. In triple-negative breast cancer (TNBC), the efficacy of CDK4/6i remains uncertain. Our study shows that the selective CDK2 inhibitor BLU-222, while effective alone, enhances synergistic activity when combined with CDK4/6i in resistant HR+/HER2- and TNBC models,…

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43 The role of low-complexity repeats in RNA-RNA interactions and a deep learning framework for duplex prediction

推荐理由: 该研究创新性地揭示低复杂度重复序列（LCRs）作为RNA互作网络的关键枢纽，其发现为理解RNA调控机制提供了新视角，对血液疾病中异常RNA互作的研究具有潜在借鉴价值。

摘要: RNA-RNA interactions (RRIs) are fundamental to gene regulation and RNA processing, yet their molecular determinants remain unclear. In this work, we analyze several large-scale RRI datasets and identify low-complexity repeats (LCRs), including simple tandem repeats, as key drivers of RRIs. Our findings reveal that LCRs enable thermodynamically stable interactions with multiple partners, positioning them as key hubs in RNA-RNA interaction networks. These RRIs appear to be important for several…

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44 Harnessing P<sup>V</sup>-oxirene for the modular synthesis of α-Oxy carbonyls

推荐理由: 该研究提出了一种新颖通用的铜催化羰基1,2-转位反应，突破了α-氧代羰基化合物的合成瓶颈，其方法学创新为开发血液疾病相关药物先导化合物提供了有价值的合成工具参考。

摘要: α-Oxy carbonyl compounds, characterized by their distinct electronic and topological properties, represent highly versatile building blocks and modifiers for functional molecules. Despite their potential, the synthetic challenges associated with these compounds have limited their exploration in chemical biology and drug discovery. In this study, we address this limitation by introducing a general Cu-catalyzed three-component carbonyl 1,2-transposition reaction, which proceeds via a P^(V)-oxirene…

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45 Real word data with no evidence for additional left atrial substrate modification at first PVI procedure in patients with persistent atrial fibrillation - Results from the German Ablation registry

推荐理由: 该研究基于前瞻性注册数据的回顾性分析，方法学较为严谨，结论对心房颤动消融术式选择具有明确参考价值；虽非直接针对血液疾病，但其对血栓栓塞风险评估及抗凝治疗策略的间接影响，值得血液科医生关注。

摘要: CONCLUSIONS: The retrospective analysis of prospectively collected data from the German Ablation registry found no evidence that additional substrate modification beyond pulmonary vein isolation during the first ablation procedure for persistent atrial fibrillation improves outcomes.

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46 Exploring Placental Dysfunction: Models of Extracellular Vesicle Action in Preeclampsia

推荐理由: 该文章聚焦于子痫前期这一重大妊娠并发症，虽非直接研究血液疾病，但其涉及的血管内皮损伤、凝血功能异常等病理机制对研究妊娠相关血液系统并发症有重要借鉴意义，且社会关注度和临床需求极高。

摘要: Preeclampsia is a life-threatening pregnancy complication, resulting in greater than 70,000 maternal deaths and over 500,000 fetal and neonatal deaths worldwide each year. Despite its prevalence and devastating consequences, there is still no medical therapy to halt disease progression and thus a vital need for novel treatments remains. The urgent demand for effective therapies for preeclampsia first necessitates a deeper understanding of the pathogenesis that underpins the condition….

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47 Second Harmonic Generation Imaging as a Virtual Biopsy for Upper Extremity Nerve Injuries: A Cadaver Study

推荐理由: 该研究首次将SHG显微技术用于人体神经组织成像，方法学创新性强，成像细节突出，虽不直接涉及血液疾病，但其高分辨胶原成像技术对研究骨髓基质或血管相关病变有潜在借鉴价值。

摘要: CONCLUSIONS: This is the first study to visualize human nervous tissue using SHG microscopy. Second Harmonic Generation imaging offers detailed visualization of all collagenous substructures of peripheral nerves and detects structural damage, like epineurial collagen-disorganization, and exposure of individual fascicles in unprecedented detail.

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48 The International Study Group for Pancreatic Surgery (ISGPS) Definition and Classification of Postpancreatectomy Mortality

推荐理由: 该研究虽聚焦胰腺手术，但其对术后死亡率的标准化定义与分类方法，可为血液病研究中并发症（如出血、血栓）的评估体系提供重要借鉴。

摘要: OBJECTIVE: The International Study Group of Pancreatic Surgery (ISGPS) aimed to uniform the definition and classification of mortality following pancreatic resections, to guide strategies for reducing preventable deaths and standardize reporting.

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49 QuAPPro: an R shiny app for quantification and alignment of polysome profiles

推荐理由: 该工具提升了多核糖体图谱分析的效率和可重复性，对血液疾病研究中翻译调控机制的探索具有实用借鉴价值。

摘要: CONCLUSIONS: This user-friendly tool does not only speed up the analysis of polysome profiles but also facilitates reproducibility and documentation of the process, without the need for programming abilities or commercial software.

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50 Streamlined resource-efficient plasma amyloid-beta mass spectrometry assay has improved biomarker performance in preclinical Alzheimer’s disease

推荐理由: 该研究开发的高效、低成本的IP-MS方法不仅优化了

摘要: Plasma amyloid-β (Aβ) peptides, alone or in ratio with p-tau217, show strong potential as Alzheimer’s disease biomarkers. While immunoprecipitation-mass spectrometry (IP-MS) is the preferred method for plasma Aβ quantification, current assays are resource- and time-intensive. Here, we developed a streamlined IP-MS method using a cost-effective instrument that significantly improved the efficiency of an original assay by incorporating a single immunoprecipitation step, an optimized buffer system,…

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51 A Magnon-photon interface based on Van der Waals Magnetic semiconductor

推荐理由: 该研究在自旋光子耦合与相干调控方面具有前沿性和方法论参考价值，虽不直接关联血液疾病，但其在磁有序材料中的光学调控思路可为血液学中磁性纳米颗粒或细胞成像技术提供跨学科启发。

摘要: Coherent control of light with electron spins represents a fundamental goal across multiple research fields, such as opto-spintronics, chiroptics, quantum information. In a magnetic-ordered material, coherent magnon gives rise to temporally and spatially modulated spin structure that may stimulate intriguing spin-photon interactions as well as opto-spintronic applications. However, the development of such magnon-photon interface is hampered due to the lack of magnetic photonic structure that…

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52 A comprehensive catalogue of receptor-binding domains in extracellular contractile injection systems

推荐理由: 该研究对eCIS尾丝蛋白进行了大规模基因组学分析，系统揭示了

摘要: Extracellular contractile injection systems (eCISs) are bacteriophage tail-derived toxin delivery complexes in prokaryotes. They play roles in microbial interactions with hosts, using tail fiber proteins for target cell binding. Here, we present a comprehensive analysis of eCIS tail fiber genes in bacterial and archaeal genomes, providing insights into their remarkable diversity, target cells, functional adaptations, and evolutionary dynamics. We identified 3445 eCIS tail fiber proteins encoded…

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53 Identification of Chlamydia pneumoniae and NLRP3 inflammasome activation in Alzheimer’s disease retina

推荐理由: 该研究通过视网膜病理与蛋白组学揭示了阿尔茨海默病中肺炎衣原体感染与NLRP3炎症小体通路的新关联，其视网膜检测方法对神经退行性疾病机制研究具有可借鉴性。

摘要: Chlamydia pneumoniae is an intracellular bacterium implicated in Alzheimer’s disease (AD), but its role in retinal pathology and disease progression is unclear. Here we identify Chlamydia pneumoniae inclusions in the retina, showing higher burden in AD retina and brain, increasing with APOEε4, disease stage, and cognitive deficit. Retinal and cortical proteomics reveal bacterial-infection and related NLRP3-inflammasome pathways. Retinal NLRP3 is elevated in mild cognitive impairment and…

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54 Biophysical characterization of Eag chaperones suggests the mechanism of effector transmembrane domain release

推荐理由: 该文章在细菌分泌系统分子机制研究上具有高度创新性和严谨性，虽然不直接研究血液疾病，但其揭示的毒力效应蛋白分泌机制可为研究某些细菌感染相关的血液疾病（如败血症）提供分子层面的借鉴思路。

摘要: The type VI secretion system (T6SS) is a dynamic protein nanomachine found in Gram-negative bacteria that secretes toxic effectors into prey-cells. For secretion, effectors require chaperones or adaptors for proper loading onto the T6SS. Effector associated genes (Eags) are a family of T6SS chaperones that stabilize N-terminal transmembrane domains (TMDs) found in thousands of effectors. Eags are essential for secretion and inhibit effector TMDs from prematurely adopting a membrane-penetrative…

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55 Reversible bismuth reduction-driven dark photoelectrochemistry

推荐理由: 该研究提出了“暗增强光电化学”新概念，在材料设计与机制创新上具有较高原创性，虽与血液疾病无直接关联，但其界面调控思路可能为生物传感或相关医学检测技术提供跨学科借鉴。

摘要: Current research in photoelectrochemical (PEC) systems focuses on designing photoelectrodes with light-enhanced charge transfer efficiency at the solid-liquid interface, which leads to the oxidation of various reductant molecules at the anode, posing a challenge in terms of selectivity. In this study, we introduce an original concept of dark-enhanced PEC system (dark-PEC) based on bismuth oxybromide (BiOBr)-photocathode. The reversible bismuth reduction causes the passivation and regeneration of…

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56 Hierarchical maturation of structural brain connectomes from birth to childhood

推荐理由: 该研究虽非直接针对血液疾病，但其采用的连接组学图谱与机器学习方法具有高度的方法学创新性，可为血液系统疾病的网络生物学研究提供重要的跨学科方法借鉴。

摘要: The postnatal white matter connectome undergoes profound reorganization, yet the topological principles governing its spatiotemporal maturation remain largely unknown. Using connectome mapping, machine learning, and neurobiological annotation, we show hierarchical network development from birth to childhood and its association with neurobiological signatures. We identify two cardinal topological transformations that change rapidly during infancy and continue to refine into childhood, as…

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57 Dynamic single-cell transcriptomics reveals lsamp-guided neural network formation in male S. japonicum driving female reproduction

推荐理由: 该研究利用前沿的单细胞转录组技术构建了日本血吸虫的高分辨率细胞图谱，虽非直接研究血液疾病，但其在寄生虫发育生物学上的方法学创新可为血液寄生虫研究提供重要技术借鉴。

摘要: Sexual maturation and egg production in schistosomes require sustained male-female pairing, yet the cellular dynamics underlying this process remain poorly understood. Here, we generate a high-resolution single-cell transcriptomic atlas of 104,671 cells from male and female Schistosoma japonicum across four developmental stages, identifying 76 distinct cell populations and mapping lineage trajectories in reproductive and neural systems. Notably, we discover a male-specific neuron subtype - N4.3…

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58 Age-Related Risk After Kidney Transplantation: A Comprehensive Analysis of Infection Burden, Graft Outcomes, and Mortality

推荐理由: 该研究虽聚焦肾移植，但其对老年患者感染负担与预后的深度分析方法，为血液科研究免疫抑制相关并发症（如感染、移植后造血异常）提供了方法学借鉴。

摘要: Given the increasing number of kidney transplantation in elderly recipients, understanding age-specific risks is essential for optimized post-transplant care. We analyzed 572 kidney transplant recipients from the DZIF Transplant Cohort (2012-2023), stratified by age: <40 (n = 146), 40-60 (n = 279), >60 years (n = 147). Outcomes included infection burden, graft outcomes, and mortality over a median follow-up of 5 years. Multivariable Cox models with inverse probability weighting, adjusted for…

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59 Effect of a behavioral counseling for adoption and maintenance of a physically active lifestyle on long-term mortality in people with type 2 diabetes: post hoc analysis of the Italian Diabetes and Exercise Study_2

推荐理由: 该研究虽以糖尿病为主要对象，但其对运动干预与长期死亡率关系的探讨，可为血液疾病（如与炎症、凝血功能相关的血液病变）的非药物干预研究提供间接的机制参考和临床思路借鉴。

摘要: An inverse relationship between physical activity and mortality has been observed in epidemiological surveys, but not in randomized clinical trials. This post hoc, not pre-specified analysis of the Italian Diabetes and Exercise Study_2 randomized clinical trial assesses the effect of a physical activity-based counseling intervention on long-term mortality in people with type 2 diabetes. Three hundred physically inactive and sedentary individuals are recruited in three outpatient diabetes clinics…

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60 Impact of a Clinical Valve Coordinator on Hospital Length of Stay and Patient Outcomes: Results From the BENCHMARK Registry

推荐理由: 该研究展示了多学科团队协作中加入专职协调员对改善患者就医流程与体验的明确价值，其管理模式对血液病等需要长期综合治疗的专科具有可借鉴的团队协作参考意义。

摘要: CONCLUSIONS: The addition of a CVC to the multidisciplinary team and their sustained contributions to processes of care align with the implementation of Benchmark practices, significantly decrease the health service requirements of TAVI patients, and are associated with improved patient-reported experiences.

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61 LINC00476 cooperates with ARIH2 and suppresses pancreatic cancer progression by inducing VIM ubiquitination

推荐理由: 该研究在肿瘤分子机制层面解析深入，揭示了LncRNA通过招募E3连接酶调控蛋白稳定性的新路径，其严谨的机制研究范式对血液肿瘤领域探索非编码RNA功能具有较高的借鉴价值。

摘要: CONCLUSION: Our data suggest that LINC00476 enhances VIM ubiquitination and degradation by recruiting ARIH2, highlighting the role of LINC00476 in PDAC progression and indicating that the overexpression of LINC00476 or ARIH2 may serve as a promising therapeutic strategy for PDAC.

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62 Mechanistic insights into fatty acid odor detection mediated by class II olfactory receptors

推荐理由: 该研究通过冷冻电镜解析了II类嗅觉受体的结构机制，方法学严谨、创新性高，虽然不直接研究血液疾病，但其对疏水性配体识别机制的揭示可为血液中脂类代谢物信号转导研究提供结构生物学借鉴。

摘要: Smell is one of the fundamental senses mediated by thousands of odorant receptors (ORs). How hydrophobic and volatile odor molecules are recognized by class II ORs remains elusive. Here, we present cryo-electron microscopy (cryo-EM) structures of class II OR Olfr110 complexed with the unsaturated fatty acid metabolite (UFAM) PL45, Gs, and cons-OR5. The structural study revealed an unusually large hydrophobic pocket accommodating PL45 and the endogenous agonist 12(S)-hydroxyeicosapentaenoic acid…

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63 Neural crest cell-derived DKK1 and NEDD4 modulate Wnt signalling in the second heart field to orchestrate outflow tract development

推荐理由: 该研究在发育生物学领域揭示了DKK1-Wnt信号轴调控心脏前体细胞行为的新机制，其信号通路研究思路对造血干细胞调控等血液学基础研究具有潜在借鉴价值。

摘要: Cardiac outflow tract morphogenesis requires coordinated interactions between multiple cell populations and is dependent on the contribution of cardiac progenitors from the second heart field. While neural crest cells have been proposed to impact second heart field development, how they regulate progenitor behaviour remains unclear. Here, we discover neural crest cells are a primary source of Dickkopf-1 (DKK1) which modulates Wnt signalling activity in the second heart field to influence the…

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64 Dual-functional quorum sensing signal synthases DspII and DspI coordinate virulence switch in Pseudomonas aeruginosa

推荐理由: 该研究鉴定出一种新型细菌运动-生物膜转换调控酶，对理解慢性感染机制有创新贡献，可为血液科感染防控（如导管相关血流感染）提供潜在分子靶点参考。

摘要: The transition between motility-associated acute infection and biofilm-associated chronic infection is crucial for bacterial proliferation and survival within hosts. This process of transition can be influenced by various host and environmental cues, however, whether bacteria could actively modulate the switch remains poorly understood. Here we identify a novel enoyl-CoA hydratase/isomerase, PA0744 (designated as DspII), which is co-upregulated with the previously characterized enoyl-CoA…

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65 Scalable and programmable topological transitions in plasmonic Moiré superlattices

推荐理由: 该文章在波前工程与拓扑相变领域展示了高度创新的方法学，虽然不直接涉及血液疾病，但其在纳米光子学与可编程超材料方面的技术突破，可能为未来高精度生物传感或细胞成像技术提供跨学科借鉴思路。

摘要: Topological transitions are fundamental phenomena in electronics, photonics, and quantum technologies. However, their scalability and tunability are constrained by material properties or structural rigidities. Here, we demonstrate that plasmonic Moiré superlattices offer a platform for programmable, large-range topological transitions via wavefront engineering. By tailoring the phases of elementary evanescent waves in hexagonal systems, we create Moiré lattices of optical skyrmions, whose…

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66 Electrical Synapses Contribute to Sleep-Dependent Declarative Memory Retention

推荐理由: 该研究虽设计严谨，但主要聚焦于神经科学领域，与血液疾病的直接关联较弱；不过其中关于甲氟喹（mefloquine）的作用机制可为血液科医生提供药物副作用的参考背景。

摘要: Sleep supports memory formation by neurophysiological mechanisms that are yet to be fully uncovered. We investigated the contribution of the direct coupling of neurons via electrical synapses (gap junctions). The administration of mefloquine (250 mg p.o. vs. placebo), an antimalarial, which blocks electrical synapses, to healthy young men before nocturnal sleep impaired the retention of word pairs learned before drug administration and disrupted the coupling of sleep spindles to EEG slow…

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67 Strengthening translational preclinical research through confirmatory multi-laboratory studies

推荐理由: 该文章系统探讨了临床前验证研究的重要性，虽然未直接聚焦血液疾病，但其提出的提高临床转化成功率的框架与方法对血液学领域的转化研究具有普遍借鉴意义。

摘要: Successful translation of promising preclinical findings into clinical application remains challenging. To address the rising concerns of failing clinical trials and the resulting economic, social, and ethical consequences, preclinical confirmatory studies have been proposed to generate sufficiently robust evidence for guiding the decision-making process. In a unique funding call, 17 studies in Germany aimed to confirm exploratory findings across various biomedical research fields in a…

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68 A negative binomial latent factor model for paired microbiome sequencing data

推荐理由: 该文章提出的多部位微生物组数据潜在因子模型方法学创新性强，虽然不直接针对血液疾病，但其处理多源关联数据的思路对血液病多组学研究具有可借鉴的方法学参考价值。

摘要: CONCLUSIONS: We propose a latent factor model for microbiome sequencing data collected from multiple sites. It captures the presumptive underlying cross-site associations without compromising estimation accuracy or inference efficiency in the absence of such associations. In addition, our proposed model improves predictive performance by enabling the prediction of microbial abundance at one site based on observations from another. We also provide an extended framework that allows for clustering…

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69 A novel dual histone mark reader ZCWPW2 regulates meiotic recombination through lactylation and transcriptional regulation in humans and mice

推荐理由: 该研究深入探讨了减数分裂中表观遗传调控的新机制，虽然与血液疾病无直接关联，但其对染色质修饰与细胞分裂过程的解析可为造血干细胞分化研究提供可借鉴的思路。

摘要: Meiotic recombination ensures accurate chromosome segregation and genetic diversity during gametogenesis, and its disruption leads to infertility. The dual histone methylation writer-reader system, in which PRDM9 deposits H3K4me3 and H3K36me3 marks at nucleosomes to define recombination hotspots and ZCWPW1 acts as a reader recognizing these marks, is essential for meiotic recombination. However, the regulatory mechanisms of this system remain unclear. Here, we showed that deficiency of ZCWPW2…

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70 A trans-synaptic IgLON adhesion molecular complex directly contacts and clusters a nicotinic receptor

推荐理由: 该研究在突触受体聚集机制上提供了创新的细胞外相互作用视角，方法严谨，虽非直接针对血液疾病，但其受体靶向机制对血液细胞信号转导研究具有潜在借鉴意义。

摘要: The clustering of neurotransmitter receptors at appropriate postsynaptic sites is essential for controlling synaptic transmission. While most known mechanisms involve receptor binding with cytoplasmic scaffolds, recent evidence highlights the importance of extracellular interactions that directly target receptors. Using Caenorhabditis elegans, we identified a trans-synaptic complex that involves RIG-5 and ZIG-8, two adhesion molecules of the immunoglobulin (Ig) superfamily and orthologous to…

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71 Stress vesicles link epidermal mechanotransduction to stem cell differentiation

推荐理由: 该研究虽聚焦皮肤干细胞力学响应，但其揭示的机械力影响干细胞命运的机制，为血液系统（如骨髓造血微环境力学调控）提供了可类比的研究思路与方法学借鉴。

摘要: The skin exhibits extraordinary plasticity, enabling it to adapt to mechanical changes in the environment. While transient deformations are accommodated without lasting structural effects, sustained mechanical stress induces durable tissue changes. To investigate if these responses are mediated by shifts in epidermal stem cell fate, we employed two-photon intravital imaging to visualize epidermal cells in live skin subjected to acute mechanical forces. Mechanical force triggered the formation of…

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72 Marvellous microbes, memory and the multiverse: Books in brief

推荐理由: 由于未提供摘要，无法评估文章的具体内容、方法和价值，因此所有评分均为中性基准分。建议提供摘要以便进行准确评价。

摘要: No abstract

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73 Resurrected nitrogenases recapitulate canonical N-isotope biosignatures over two billion years

推荐理由: 该研究通过重建远古固氮酶基因探讨氮同位素分馏机制，方法严谨且具创新性，可为早期地球生命活动提供重要线索，但与血液疾病直接关联较弱，仅对涉及氮代谢的极少数血液学研究有间接参考价值。

摘要: Nitrogen isotope fractionation (ε^(15)N) in sedimentary rocks has provided evidence for biological nitrogen fixation, and thus primary productivity, on the early Earth. However, the extent to which molecular evolution has influenced the isotopic signatures of nitrogenase, the enzyme that catalyzes the conversion of atmospheric nitrogen (N₂) to bioavailable ammonia, remains unresolved. Here, we reconstruct and experimentally characterize a library of synthetic ancestral nitrogenase genes,…

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74 Methodology and Epidemiologic Data of the Second International Spinal Cord Injury (InSCI) Community Survey

推荐理由: 该文章虽聚焦脊髓损伤的全球医疗应对，与血液疾病无直接关联，但其基于大规模社区调查的数据分析方法及医疗资源优化思路，可为血液病卫生政策研究提供方法学参考。

摘要: CONCLUSIONS: The rising incidence of SCI worldwide requires tailored health care, rehabilitation, and support systems. Data from the second InSCI community survey will be instrumental to identify gaps in health care provision, inform targeted interventions, and optimize resource allocation.

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75 Age and sex as risk factors for health-related quality of life outcomes in patients with glioma: a CODAGLIO 2.0 analysis

推荐理由: 该研究主要针对神经胶质瘤患者的生活质量，与血液疾病直接相关性较弱，但其中关于患者报告结局（PRO）的评估方法和“临床相关性差异”的分析思路，可为血液肿瘤或慢性血液病患者的生活质量研究提供方法学参考。

摘要: CONCLUSION: Patients with glioma have clinically relevant worse HRQoL compared to the general population. There are statistically but not clinically significant associations between age, sex, and certain HRQoL scales.

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76 Prognostic value of anemia plus thrombocytopenia in primary myelofibrosis

推荐理由: 由于未提供摘要内容，无法进行实质性评估，但考虑到该请求明确指向血液病研究领域，若原文内容相关，或对血液专科有一定参考价值。

摘要: No abstract

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77 Additive-specific modulation of non-classical nucleation pathways

推荐理由: 该文章在结晶机理研究方法上具有较高的技术参考价值，但其研究对象（硅酸盐水泥水化物）与血液疾病直接关联较弱，仅可能在生物矿化或病理钙化等间接领域提供有限思路。

摘要: Additives, comprising small quantities of organic and inorganic molecules, are crucial for controlling the crystallisation of various (bio)materials; however, their mechanisms remain poorly understood. By integrating in situ high-energy X-ray scattering with potentiometric titrations, we examine the impact of industrially relevant additives on the nucleation pathways of portlandite and gypsum. While both minerals undergo multistep nucleation, portlandite transitions gradually from a disordered…

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78 Asymmetrical covalent organic framework mixed matrix membranes for highly efficient gas separation

推荐理由: 该文章在材料科学与膜分离技术领域方法创新、工艺严谨，但内容主要针对气体分离膜制备，与血液疾病研究无直接关联，血液病领域可借鉴性极低。

摘要: The asymmetrical and seamless Covalent organic framework (COF)-mixed matrix membranes (COF-MMMs) extending over the centimeter scale for highly efficient H(2)/CO(2) separation are fabricated via a non-solvent induced phase separation (NIPS)-triggered in situ interfacial polymerization (IP) strategy. Here, a dense and ultrathin COF membrane (15-30 nm thick) is fabricated on the surface of the Polyether sulfone (PES) skin layer, and isolated COF nanocrystals (4-8 nm) are formed and highly…

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79 A Survey of Single-Use and Reusable Surgical and Anaesthetic Devices in Victorian Public Health Services

推荐理由: 本文主要关注医疗采购与环境可持续性，虽涉及医疗设备使用，但与血液疾病无直接关联，对血液学研究的可借鉴性有限，适合关注医疗系统绿色转型的政策研究者阅读。

摘要: CONCLUSIONS: Most (8/9) public health services in Melbourne surveyed utilise mostly single-use devices in perioperative settings. This audit provides a snapshot where reusable alternatives are available to influence future procurement. As the Victorian healthcare system rapidly transitions to renewable energy sources of electricity, there are considerable procurement opportunities to reduce scope three greenhouse gas emissions from healthcare.

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80 Breaking the cell wall for efficient DNA delivery to diatoms

推荐理由: 该文章在微藻遗传工程方法学上具有创新性和技术参考价值，但其研究对象与血液疾病无直接关联，对血液学研究可借鉴性有限，仅建议关注基因编辑工具

摘要: Diatoms produce 20% of the world’s fixed organic carbon yet remain underutilized as cell factories due to limited genetic engineering tools. Here, we present optimized electroporation and polyethylene glycol (PEG) transformation methods for the model diatom Phaeodactylum tricornutum, enabling delivery of DNA and Cas9 ribonucleoprotein complexes with high efficiency. Transformants are recovered with as little as 1 ng of DNA, and linear or circular episomes as large as 55.6 kb are successfully…

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